The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and Pölöskei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.

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Aims: Evaluate the efficiency of sources and dosages of 6-benzyladenine in the chemical thinning of the apple tree 'Fuji Suprema', as well as possible histological changes in the fruits produced, in the Midwestern region of the state of Santa Catarina. Study Design: The experiment was arranged in a randomized block design and replicated six replications. Place and Duration of Study:The experiment was carried out in the municipality of Caçador, Brazil (latitude 26º46 'S, longitude 51º W, altitude 960 meters), during the growing season of 2016/2017. Methodology: Two commercial products based on 6-BA (Benzyladenine), Exilis ® and Maxcel ® , both with 2% BA in their composition, were tested and applied individually in post-flowering period (in fruits of 5-10 mm), which were compared with plants with no thinning and manual thinning. Treatments were as follows: Control (no thinning); manual thinning; Exilis ® (1.5, 3.0, 4.5 and 6.0 L Gabardo et al.; JEAI, 32(6): 1-9, 2019; Article no.JEAI.48031 2 ha -1 ) and Maxcel® 4.5 L ha -1 ), using a spray volume equivalent to 1000 L ha -1 . The fruit set and the number of fruit per cm 2 branch, was reduced in treated plants, regardless of the concentration of BA or formulation used. The productivity ranged from 38 to 56.5 t ha -1 in the treatments of Exilis ® , 60.5 t ha -1 in manual scaling and 24.5 t ha -1 in the Maxcel ® 4.5 L ha -1 , characterizing excessive thinning. BA is efficient in fruit thinning in 'Fuji Suprema' apple trees, being the reduction of fruiting, and increase of fruit size, proportional to the applied concentration. There may be differentiated efficiency of the product by its formulation, even though they have concentrations of active ingredient equivalent. Exilis® was efficient in thinning of apple "Fuji Supreme", when applied to fruit 5 to 10 mm in diameter reduced the need for manual thinning, without causing toxicity. BA can promote the increase in the cell density of fruits produced in proportion to the applied concentration. Original Research Article

Aims: Evaluate possible alterations in the development, anatomy and quality of fruits from 'Maxi Gala' and 'Fuji Suprema' apple plants treated with Metamitrom (MM) and Benzyladenine (BA) alone or in combination, applied at different stages of fruit development in the Midwestern region of the state of Santa Catarina. Study Design: The experiment was arranged in a randomized block design with five replicates. Place and duration of Study: The experiment was carried out in the municipality of Caçador-SC, Brazil, during the growing season of 2016/2017. Methodology: Two products, BA (with hormonal action) and MM (photosynthesis inhibitor) were applied individually or in a tank mix in post-flowering periods (in fruits of 5-10 mm and 15-20 mm in equatorial diameter), which were compared with plants with no thinning and manual thinning of both cultivars. Treatments were as follows: Control (no thinning) MM, BA, MM + BA (in fruits of 5-10 mm in diameter); MM, BA, MM + BA, manual thinning (in fruits of 15-20 mm in diameter). The phenological stage of F2 (Full flowering (FF) was observed on September 28, 2016 for both cultivars. Evaluations of the anatomical characteristics of fruits at the time of harvest did not show any alteration in cell size (mean cell area - μm2) and cell density (number of cells per fruit section area); the mean fruit mass was higher in MM 350 mg L-1 (5-10 mm), MM 350 mg L-1 + BA 40 mg L-1 (5-10 mm), and MM 350 mg L-1 (15-20mm) treatments. In the 'Fuji Suprema' cultivar, except for control treatments, MM 350 mg L-1 (5-10mm) and BA 80 mg L-1 (5-10mm), a significant reduction in production was observed (kg plant-1 and fruit-1). While in 'MaxiGala', all the thinning treatments reduced yield per plant. The cultural thinning practice, fruit load management, adapting the number of fruits according to plant size is the most important factor that producers are able to influence. Thinning reduces fruit load in the plant, which allows remaining fruits the possibility of greater growth and size gain. The combination of MM + BA is efficient in the thinning of apple fruits, especially when applied at the beginning of fruit development (5-10 mm in diameter). The application of chemical thinners, such as MM and BA, may influence the early stages of fruit development.

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