Background: Among new therapies emerging in the medical field, the use of platelet-rich plasma (PRP) in human reproduction has not yet been explored. Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in the rabbit model. Material and methods: A total of 30 male rabbits were recruited in this study. They were allocated into two groups (15 in each group) to receive an injection of PRP (PRP Group), or normal saline (Control Group) Results: there were statistically significant differences in Means of germinal layer width, Leydig cell number, and Sertoli cell number was significantly higher in the PRP group compared to that in the control group ( P ≤ 0.05). The PRP group had a higher means of sperm concentration and normal morphology compared with the control groups (P ≤ 0.05). Conclusion: the platelet-rich plasma is found to have a good potential effect on the testicular tissue that improved the histological and functional aspects and could be considered a promising future treatment for hypogonadism status in many disorders. Keywords: PRP, reproductive function, testis, Sertoli cells, Leydig cells, the height of the germinal layer KEY WORDS: Platelet-Rich Plasma (PRP), Tissue Progenitor Cells, Testis.