Background Preservation of bite marks evidence has always been a major problem in forensic odontology due to progressive loss of details as time passes. The use of 2D photographs has been widely used to document forensic evidence and preserving bite marks; however, there are limitations to this method. This study aims to measure the accuracy of the 3D scanned image in comparison to 2D photograph registration of experimental bite marks. Thirty volunteers performed self-exertions of a bite mark on the respective forearm of subjects. A 2D photograph and 3D scanned image was immediately registered following bite mark exercise using a conventional camera and Afinia EinScan-Pro 2X PLUS Handheld 3D Scanner, respectively. The outlines of the bite mark were transformed into a polygonal shape. Next, the polygonal approximation analysis was performed by an arbitrary superimposition method. The difference between surface areas of both images was calculated (2D photographs ̶ 3D scanned images). Results A paired t test was used to measure significance with α = 0.05. The mean surface area of 2D photographs and 3D scanned images is 31.535 cm2 and 31.822 cm2, respectively. No statistical difference was found between both mean surface areas (p > 0.05). The mean error (ME) is 0.287 ± 3.424 cm2 and the mean absolute error (MAE) is 1.733 ± 1.149 cm2. Conclusion Bite marks registered with the 3D scanned image are comparable to the standard 2D photograph for bite mark evaluations. The use of a 3D scan may be adopted as a standard operating procedure in the forensic application, especially for evidence preservation.