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Electrochemically polymerised riboflavin (PRf) film was applied in development of glutamate and hypoxanthine biosensors using appropriate oxidases as recognition elements. PRf served as an enzyme immobilisation matrix composed of natural monomer and as a redox mediator. The PRf film was deposited on glassy carbon electrode (GCE) or on the laser‐designed microstructures of the chitosan‐graphene oxide (Chit‐GO) film deposited on glass slide, which served as a chip sensor. The redox polymer was stable and efficient mediator, however, Chit‐GO was placed on the top of PRf/GCE in order to increase the response signal. Such an approach allowed to extend the stability of glutamate and hypoxanthine biosensors to at least 1 month and 15 days, respectively. The chip microelectrode was a single use disposable biosensor due to insufficiently good Chit‐GO adhesion on the top of the cover‐glass. The glutamate microbiosensor had extremely high sensitivity of 16 μA cm−2 μmol L−1.
Conventional three-dimensional optics requires curvature to control the wave front of light thus making it difficult to reduce the size of the optical systems. Furthermore, for correction of optical aberrations, complex optical systems comprising more than one lens are used. This adds additional bulk, mass and complexity to the optical systems. Recent development in diffractive optics has enabled new thin lightweight optical elements such as metalenses. We introduce resonant laser printing technique as a flexible photo-thermal technology for metalens fabrication with the ability to control the light with microscale precision. Our laser printed metalenses can be integrated in bio-sensors, bio-imaging systems, and optofluidical devices.
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