Two triterpenoids, taraxerone and tricadenic acid A were isolated from the methanol extract of the outer bark of Schleichera oleosa available in Darjeeling foothills. A preliminary study on their antimicrobial activities was also performed against some fungal and bacterial species. The structure of these compounds was determined by means of chemical characterisation and IR, NMR spectral data.
The study reports chemically characterised Myristica fragrans essential oil (MFEO) as plant based food preservative against fungal and aflatoxin B 1 (AFB 1 ) contamination of scented rice varieties. The chemical profile of MFEO revealed elemicin (27.08%), myristicine (21.29%) and thujanol (18.55%) as major components. The minimum inhibitory and minimum aflatoxin inhibitory concentrations of MFEO were 2.75 and 1.5 mg/ml, respectively. The MFEO was efficacious against a broad spectrum of food deteriorating fungi. MFEO caused decrease in ergosterol content of fungal plasma membrane and enhanced leakage of cellular ions, depicting plasma membrane as site of action. The MFEO caused reduction in cellular methylglyoxal content, the aflatoxin inducer. This is the first time report on MFEO as aflatoxin suppressor. The essential oil may be recommended as plant based food preservative after large scale trials and reduction in methylglyoxal suggests its application for development of aflatoxin resistant varieties through green transgenics.
Water, the central molecule of life, plays a profound role in a number of plant life processes ranging from photosynthesis to macromolecular interaction through hydrophobic bond. Due to imbalances in natural status of the different physiological, environmental conditions and during natural calamities, plants are exposed to either deficit of water (i.e. drought) or excess of water (i.e. flooding). Both of these conditions lead to water stress on plants which in turn results in disruption of agriculture and food supply in different parts of the world. In this chapter, a brief idea on the causes, indicators, responses and adaptation processes to the water stress in plants and the associated molecular mechanisms has been presented. In this chapter, the stresses related to water are expressed as "drought". The cellular and molecular responses of plants to water stress have been studied intensively throughout the world. Understanding the mechanisms by which plants perceive water stress and transmit the subsequent signals to cellular machinery and modulate expression of genes and their products to activate adaptive responses is of fundamental importance to plant biology. Knowledge about water-stress signal transduction is therefore vital for continued development of rational breeding and transgenic strategies to improve stress tolerance in crops. Factors controlling waterstress conditions alter the normal equilibrium and lead to a series of morphological, physiological, biochemical and molecular changes in plants which adversely affect their growth and productivity. However, plants also have developed innate adaptations to water-stress conditions
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