A gram-negative bacterium which was capable of oxidizing reduced inorganic sulfur compounds was isolated from agricultural soil and designated BI-42. This new isolate grew on a wide range of organic substrates but was not able to grow autotrophically and lacked ribulose 1,s-bisphosphate carboxylase, a key enzyme of carbon dioxide fixation. These results suggested that strain BI-42 was a chemolithoheterotroph. Ammonia and nitrate were not used as sole nitrogen sources for growth, and strain BI-42 lacked glutamate synthase activity, which resulted in glutamate auxotrophy. The glutamate dehydrogenase activity of this organism was apparently insufficient for ammonia assimilation. On the basis of the results of additional biochemical tests, the G+C content of the DNA, the results of a respiratory ubiquinone analysis, the results of a 16s ribosomal DNA sequence analysis, the fatty acid composition, and the results of a membrane lipid analysis, strain BI-42 was identified as a phylogenetically and physiologically distinct taxon belonging to the alpha subclass of the Proteobucteria. Boseu thiooxiduns gen. nov., sp. nov. is the name proposed for this taxon.Sulfate is the end product of oxidations of reduced sulfur compounds by chemosynthetic, photosynthetic, and various heterotrophic microorganisms. Nature balances these oxidations through the biological reduction of sulfate to sulfide. Microorganisms utilize sulfur compounds for the biosynthesis of cellular material or transform these compounds as part of a respiratory energy-generating process. Most of the known sulfur-oxidizing bacteria belong to the genera Thiobacillus, Thiothrix, Beggiatoa, Thiomicrospira, Achromatium, DesuEfovibrio, Desulfomonas, Desulfococcus, and Desulfuromonas (18,55). Members of the genus Thiobacillus have been studied extensively to increase our understanding of the coupling of oxidation of reduced inorganic sulfur compounds to energy biosynthesis and assimilation of carbon dioxide (45). In addition, some phototrophic bacteria, such as members of the genus Chromatium, oxidize reduced inorganic sulfur compounds to provide reducing power for carbon dioxide assimilation during anaerobic photosynthesis (38).However, oxidation of sulfur compounds is not restricted to the true sulfur bacteria; this process also occurs in heterotrophic bacteria isolated from soil (54) and the marine environment (50, 51). Most of the heterotrophic bacteria that have such activities belong to the genera Pseudomonas (35), Mycobacterium, Arthrobacter, Flavobacterium (54), and Xanthobacter or are Escherichia coli strains (42). However, few of these heterotrophs have the ability to generate biologically useful energy from the oxidation of reduced sulfur compounds (31). An exception is Paracoccus denitrificans (6), which also grows autotrophically in the presence of thiosulfate. Only a few heterotrophic bacteria, such as Thiobacillus sp. strain Q (8) and Catenococcus thiocyclus (39), have been shown to obtain energy from the oxidation of sulfur compounds. This type of nutr...