A reciprocating oscillatory turbulent flow in a rectangular duct is investigated experimentally by making use of a laser-Doppler velocimeter, hot-wire anemometers as well as electronic digital sampling and processing equipments.The profiles of the mean velocity, the turbulence intensities, the Reynolds stress and the turbulent-energy production rate are compared for the accelerating and decelerating phases.The characteristics of such a flow are quite different from wall turbulence which is steady in the mean. In the accelerating phase, turbulence is triggered by the shear instability at a slight distance from the wall but is suppressed and cannot develop. However, with the beginning of flow deceleration, turbulence grows explosively and violently and is maintained by the bursting type of motion.The turbulent-energy production becomes exceedingly high in the decelerating phase, but the turbulence is reduced to a very low level at the end of the decelerating phase and in the accelerating stage of reversal flow. Spectra and spatial correlations for the various phases are compared. The spectral decay in the high-frequency range for the decelerating phase with high turbulence is far steeper than that of Kolmogorov's −5/3 power law, indicating remarkably high energy dissipation by high-frequency turbulence.Notwithstanding the great difference between the ensemble-averaged characteristics of the oscillatory flow and those of steady wall turbulence, its basic processes such as ejection, sweep and interactions directed towards and away from the wall are the same as those of ‘steady’ wall turbulence.

The lipid compositions of barophilic bacterial strains which contained docosahexaenoic acid (DHA [22:6n-3]) were examined, and the adaptive changes of these compositions were analyzed in response to growth pressure. In the facultatively barophilic strain 16C1, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) were major components which had the same fatty acid chains. However, in PE, monounsaturated fatty acids such as hexadecenoic acid were major components, and DHA accounted for only 3.7% of the total fatty acids, while in PG, DHA accounted for 29.6% of the total fatty acids. In response to an increase in growth pressure in strain 16C1, the amounts of saturated fatty acids in PE were reduced, and these decreases were mainly balanced by an increase in unsaturated fatty acids, including DHA. In PG, the decrease in saturated fatty acids was mainly balanced by an increase in DHA. Similar adaptive changes in fatty acid composition were observed in response to growth pressure in obligately barophilic strain 2D2. Furthermore, these adaptive changes in response were also observed in response to low temperature in strain 16C1. These results confirm that the general shift from saturated to unsaturated fatty acids including DHA is one of the adaptive changes in response to increases in pressure and suggest that DHA may play a role in maintaining the proper fluidity of membrane lipids under high pressure.

Five bacterial strains isolated from the intestine of deep sea fish were shown to produce docosahexaenoic acid (22:6n-3; DHA) at a level of 6.4 to 11.6% of total fatty acids when incubated in DHA-free medium. In all of the strains examined, other polyunsaturated fatty acids were barely detectable, except for eicosapentaenoic acid (20:5n-3). A typical strain, such as T3615, produced DHA at a concentration of about 0.8 mg/L within six days of aerobic incubation at 5 degrees C and under atmospheric pressure. The T3615 strain, belonging to the genus Vibrio, is rod-shaped, Gram-negative, motile and facultatively anaerobic.

The pressure resistances of the spores of six Bacillus strains were examined at 5 to 10؇C and were compared with their heat resistances. The pressure treatments (at 981 MPa for 40 min and at 588 MPa for 120 min) did not inactivate the spores of B. stearothermophilus IAM12043, B. subtilis IAM12118, and B. licheniformis IAM13417. However, these spores had large differences in heat resistance. The spores of B. megaterium IAM1166 were 9.3 times more pressure resistant but 246 times less heat resistant than those of B. stearothermophilus IAM11001. The spores of B. coagulans IAM1194 were activated by the pressure treatments. There was no correlation between these pressure and heat resistances.

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