The reversible formation of a glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-CP12-phosphoribulokinase (PRK) supramolecular complex, identified in oxygenic photosynthetic organisms, provides light-dependent Calvin cycle regulation in a coordinated manner. An intrinsically disordered protein (IDP) CP12 acts as a linker to sequentially bind GAPDH and PRK to downregulate both enzymes. Here, we report the crystal structures of the ternary GAPDH-CP12-NAD and binary GAPDH-NAD complexes from Synechococcus elongates. The GAPDH-CP12 complex structure reveals that the oxidized CP12 becomes partially structured upon GAPDH binding. The C-terminus of CP12 is inserted into the active-site region of GAPDH, resulting in competitive inhibition of GAPDH. This study also provides insight into how the GAPDH-CP12 complex is dissociated by a high NADP(H)/NAD(H) ratio. An unexpected increase in negative charge potential that emerged upon CP12 binding highlights the biological function of CP12 in the sequential assembly of the supramolecular complex.

AC therapy is significantly associated with reduced mortality before 3 years of age in VLBW infants with HCA, but not with neurodevelopmental outcomes, which was same as the results found in infants without HCA. AC therapy is recommended for women with suspected chorioamnionitis, as well as those without chorioamnionitis.

An efficient separation of cyclohexanol and cyclohexanone by selective complexations with the title host compounds has been achieved. Crystallographic analyses of the corresponding 1:1 complexes with the cyclohexane host derivative show a characteristic structure type which is dominated by continuous arrays of hydrogen bonds

Selective inclusion of the diequatorial isomer of 3,5-dimethylcyclohexanone and the triequatorial isomer of 3,5-dimethylcyclohexanol by 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol was observed. The crystal structure of these complexes was studied.

The title host compound forms crystalline inclusion complexes with a wide variety of organic guest molecules. X-Ray analysis of its 1:1 complex with phenol shows that the two molecular components are held together by a O–H···O hydrogen bond of length 2.59(1) Å.