An effective way to overcome shortage of donors in liver transplantation (LTx) is to consider such from non-heart-beating donors (NHBDs). We investigated how a liver graft should be treated before and/or after procurement for successful LTx from an NHBD. Porcine LTx was performed with FR167653 (FR), a dual inhibitor of tumor necrosis factor-a (TNF-a) and interleukin-1 / 3 (IL-1 p), and/or prostaglandin El (PG). Animals were allocated to an FR group (n = 4, donors and recipients were treated with FR), a PG group (n = 4, donors and recipients were treated with PG), or an FRPG group (n=4, donors and recipients were treated with both FR and PG). No recipients in the FR group and only two of four recipients in the PG group survived, whereas all recipients in the FRPG group survived. Suppression of TNF-a and IL-lp and maintenance of microcirculation are the key to successful transplantation from NHBDs.

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Poster Abstracts 5 4 8 WEDNESDAY Supplement to Transplantation July 27, 2008, Volume 86 Number 2Sfor E-1 and E-2) and 14 (p< 0,0001 for E-1 and E-2) days, if compared to the controls. After 28 days the effect remained non-signifi cant, although average values were still lower. Hydroxyproline content and concentration were measured after 7 days and show a dose dependent decline. The difference between the experimental groups and control group was signifi cant (p< 0,05), the difference between E-1 and E-2 for ileum and colon anastomoses was not signifi cant. Histological parameters did not show any apparent differences between groups. Fascial wounds were less strong in both experimental groups at all three points measured. Conclusion: Everolimus interferes with wound repair. Fibroblast collagen synthesis and deposition of new collagen fi bers are essential to the proliferation phase of normal wound repair. Most likely, impaired cellular proliferation is responsible for the effect seen here. Even after 4 weeks, wound strength appears to remain below normal. Such a prolonged effect may have clinical consequences and cause surgical morbidity. The possibility of prevention of surgical complications, or diminishing the negative effect, is an exciting incentive for further investigation.