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Analgesic effect of Keishi-Ni-Eppi-Itto-ka-Ryo-Jutsu-Bu (KNE), a traditional herbal medicine against rheumatoid arthritis, on writhing reaction induced by acetic acid was investigated in mice. KNE, composed of gypsum, Zizyphi fructus, Cinnamomum cortex, Ephedrae herba, Paeoniae radix, Glycyrrhiza, ginger, Atractylodis rhizoma and hoelen, significantly depressed the writhing reaction, while Aconiti tuber, widely used for analgesic purpose, did not affect the reaction. The depressive action of each KNE component was examined. It was found that only Ephedrae herba showed significant dose-dependent depression of the writhing reaction. When the decocted liquid of Ephedrae herba was separated to aqueous layer and benzene layer at low pH, followed by high pH, the aqueous layer showed remarkable dose-dependent depression of the writhing reaction.

The fibrinolytic activities of some commercially available urokinase preparations were compared using thrombelastgraph, and relationships between the stability of urokinase activity and additives contained in preparations, and between the specific activity and the difference of molecular weight of urokinase were studied.Results were as follows: 1) low molecular weight urokinase (LUK) was more stable than high molecular weight urokinase (HUK) . 2) HUK was kept most stable, when albumin was added.3) The stability of LUK did not depend on additives.4) When the urokinase solution was freezed and then thawed, specific activity of gelatinadded HUK was decreased to 50%, and that of albumin-added HUK to 90%.It was proved that the fibrinolytic activity of urokinase could be measured by thrombelastgraph.