Context: According to previous studies human-derived luteal phase granulosa cells are able to differentiate into other cell lineages, such as osteoblasts, chondrocytes and neurons. Aims: In this study we investigated the differentiation potential of these cells into sperm-like cells. Methods: First, we demonstrated the stemness of isolated GCs by flow cytometry and then we showed that granulosa cells can differentiate into advanced male germ cell lineages including spermatid-like cells in vitro. Follicular-phase GCs were cultured then induced by sheep testis tissue extract to differentiate into sperm-like cells, as evidenced by their expression of Gpr125, as well as VASA and DAZL, which are markers of different steps of Spermatogenesis.Key results: Mesenchymal stem cell markers were expressed in these cells. Spermatogenesis markers including DAZL,VASA and GPR125, were also expressed from the second week after induction. Morphological analysis of induced cells showed that Producing sperm- like cellsweresimilar to the normal sperm. Conclusions: Obtained results prove that isolated GCs are a kind of mesenchymal stem cells and have the capability to differentiate into sperm–like cells. Implications: This finding may represent a novel strategy for studying spermatogenesis in vitro or may help to improve assisted reproductive technology methods in the future.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.