Haplophyllum tuberculatum is an indigenous plant widely distributed in Iraq.Phytochemical investigation of this plant indicating that the plant is rich in alkaloids, fixed and volatile oils; but non about psoralen contents. Preliminary investigation indicated that this plant has furocoumarins in acceptable amount.Based on these results together with literature survey it was deemed desirable to carry out this phytochemical work with emphasis on isolation and characterization of the furocoumarins compounds.Detailed preliminary investigation of the furocoumarins content of the dried, ground whole plant has been described and the results were discussed.Thin layer chromatography of the petroleum ether (boiling point 60-80ºC) extract demonstrated the occurance of furocoumarins compounds in the plant.The extraction procedure of the plant material, the fractionation and isolation procedures of the furocoumarins are fully described.One compound was isolated and identified as Ammoidin by TLC (compared with standard), melting point (148°C), mixed melting point (148ºC) and HPLC.Spectrophotometer apparatus was used to determine exactly the amount of Ammoidin measured at 304nm at UV absorption. In Haplophyllum tuberculatum the maximum amount of Ammoidin was found in sample collecting from Daquok during June.
This work covers some phytochemical studies of a wild plant, Suaeda baccata Forsk of the family Chenopodiaceae, which covers almost all the cities of Iraq. Literature survey revealed a number of publications concerning different species of Suaeda, but none at all concerned Suaeda baccata, therefore, a research on this plant will be of important value. Several reports indicated the presence of alkaloids in some Suaeda species, thus our investigation procedures emphasized the identification and isolation of the alkaloids that might be present in this plant. Preliminary investigation on the plant extract showed the presence of the alkaloids that were tested by both Mayer’s reagent and Dragendorff’s spraying reagent, and authenticated with standard compounds by TLC using several solvent systems that proved the presence of two alkaloids, trigonelline and choline. Trigonelline crystals were subjected to tests of purity as HPLC, melting point and mixed melting point, which matches with the standard trigonelline. Extract containing choline alkaloid was identified only by TLCusing different solvent systems. Studies concerning its crystallization and identification by HPLC and melting point were left for further studies since choline is highly hygroscopic and oxidizable compound.
The dermatological preparations consist of simple or compounded bases in which one or more active ingredients are dissolved or suspended .The plant Aloe vera is widely distributed in Iraq, in addition to it's tranditional use in the treatment of occasional constipation , it is also used to assist healing of wounds ,burns and psoriasis. In this study; the amount of aloin (the major component of the plant) in the dried juice of the Iraqi plant Aloe vera is determined and was found to be 15.4% w/w and it is the major compound responsible for the antimicrobial activity of the plant.Different ointment bases were used in this study to prepare topical preparations from the dried juice of the Iraqi plant with different concentrations and were found that 4%w/w sodium carboxy methyl cellulose gel base was the best formula using comparative diffusion study through the skin. The clinical study which is carried out in this research on 61 patients with skin infections (mainly Tinea corporis and Tinea cruris) showed that 4%w/w gel base formula of aloe gave significant improvement in comparison with those treated with nystatin and hydrocortison and proved the antipruritic and antiinflammatoryaction of the dried juice of the Iraqi plant Aloe vera without any adverse effect. Finally the expiration date of aloin in the selected formula is determined and was found to be ≈ 2 years.
This study includes the detection of the anti microbial activity of both the plant extract of Suaedabaccata (Chenopodiaceae) and its constituent the trigonelline alkaloid, against several microorganisms(Staphylococcus aureus to represent one member of Gram positive bacteria, Escherichiacoli to represent one member of Gram negative bacteria and Candida albicans to represent onemember of fungi) in that trigonelline alkaloid and the plant extract, showed an antibacterial activityagainst the growth of gram positive bacteria, Staphylococcus aureus, while gram negative bacteria,Escherichia coli and the yeast, Candida albicans were resistant to both.
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