The role of healthcare workers is central to the modern healthcare system and was further highlighted during the COVID-19 pandemic situation. However, the healthcare workers did face a lot of work pressure during the pandemic which also had some negative consequences. In the present study, we investigated the effects of work stress and its five dimensions including the availability of resources, work environment, reward and incentive, functional relationship, and fear of catching COVID-19 on employee job satisfaction in the context of the public healthcare system in Oman. The sample is selected from a selected healthcare unit and data is collected using the survey-based method. The findings show that there are significant effects of two dimensions including functional relationships and work environments on healthcare job satisfaction. Based on the findings, it can be concluded that healthcare workers facing a lot of stress and this issue need greater managerial attention. Keywords: Work Stress, Job Satisfaction, Healthcare Workers, COVID-19 Pandemic.