We have extracted the mtDNA (mitochondrial DNA) from one eyebrow included in the plaster of the Antommarchi death-mask of Napoléon. The corresponding HVS1 (Hypervariable Sequence number 1) contains only one mutation, named 16184T, that is that of the Napoléon family (reference 2). That proves, in accordance with some previously established plaster particularities of the eyebrow part of the mask (reference 1) showing that it originates from the St. Helena island, that the Antommarchi death-mask of Napoléon is the nearest historical mask of the real death-mask of Napoléon.

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