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Several (6), which involved scoring the formation of lung metastases after i.v. injection of melanoma cells into immunodeficient mice (7). These studies showed that human melanoma cell lines expressing high levels of TF, which can initiate coagulation in murine as well as in human plasma, were strongly metastatic and that the metastatic potential of the cell lines could be inhibited by treatment with an anti-TF monoclonal antibody that blocks its procoagulant activity. The conclusion drawn from those results was that one or more products of the coagulation cascade mediate the metastatic effect of TF. In this report we have used a different approach to study the role of TF in promoting metastasis. Four matched sets of cloned human melanoma cell lines expressing either normal or mutant TF molecules were generated by retroviral-mediated transfections, and the metastatic potential of the transfected cells was tested in the SCID mouse model of melanoma metastasis (6 8205The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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