India has about several thousand medicinal plants which possess notable cancer curing properties. The raw usage of these plants species have been found widely in rural herbal remedies for the treatment of cancer. Many research works have been carried out concurrently in last few decades on these plants for cancer treatment and emphasized the importance of clinical support for these plants. One of such traditionally important plant is Gendarussa Vulgaris. In connection with that, the present study is aimed to give a surge of interest about cancer preventing potential of the plant G.vulgaris by in-vitro cell culture. The Chloroform, ethyl acetate and ethanol extract of the plant were used to analyse the cytotoxicity against the human cancer cell lines -human liver carcinoma cancer cell line(HepG2) and cervical cancer cell line(HeLa) by MTT assay. The results obtained showed that the ethanol extract of the plant showed better cytotoxic activity against all the tested lines and more prominent against HepG2 with the IC50 value 19.8µg/ml. The effectiveness of the ethanol extract was confirmed by comparing the experiment results with the previously analysed datas of literature. The comparison study brought a key attention on the notable cytotoxic efficacy of the plant G.Vulgaris against many cancer cell lines. However an in-vivo clinical support and isolation of active phyto constituents are needed for the reliance of the plant in chemotherapeutic use.