Crop height (Ht), heading date (Hd), and grain yield (GY) are interrelated in wheat. Independent manipulation of each is important for adaptation and performance. Validated QTL for all three collocate on chromosome 3A in the Avalon x Cadenza population with increased Ht, Hd, and GY contributed by Cadenza. We asked if these are linked or pleiotropic effects using recombinant lines and showing that Ht and Hd effects are independent. The Chinese Spring equivalent to the newly defined Ht interval contained a gene cluster involved in cell wall growth and displaying high levels of differential transcript expression. The Hd locus is larger and rearranged compared to the reference genome but FT2 (Flowering Locus T2) is of particular interest. The Hd effect acted independently of photoperiod and vernalization but did exhibit seasonal genotype x environment interaction. Recombinants were phenotyped for GY in replicated field experiments. GY was most associated with Cadenza alleles for later Hd, supporting physiological studies using the same lines proposing that ‘late’ alleles at this locus increase spike fertility and grain number (GN). The work has uncoupled height from heading and yield and shown that one of very few validated GY QTL in wheat is probably mediated by phenological variation.

Crop height (Ht), heading date (Hd), and grain yield (GY) are interrelated traits in wheat. Independent manipulation of each is important for adaptation and performance. Validated QTL for all three collocate on chromosome 3A in the Avalon x Cadenza population. We asked if these are linked or pleiotropic effects. The region was dissected using recombinants derived from Near Isogenic Lines. It was shown that Ht and Hd are controlled by independent genes. The newly defined Ht QTL interval contained a gene cluster involved in cell wall growth and displaying high levels of differential transcript expression. The Hd locus is much larger and rearranged compared to the reference genome but FT2 is a candidate of particular interest. The Hd effect was shown to act independently of photoperiod and vernalization but did exhibit genotype x environment interaction suggesting a role in ambient temperature sensitivity. It was the Hd locus that was most associated with increased GY of Cadenza alleles, supporting physiological studies proposing that ‘late’ alleles at this locus increase spike fertility and grain number. The work has uncoupled height from heading and yield and shown that one of very few validated GY QTL in wheat is probably mediated by phenological variation.HighlightThere only are three validated wheat yield QTL. Here, one of them was genetically dissected.This showed that the physiological basis of the yield effect is likely to be phenological.