Human activities involving noise emission can affect wild animals. European mink was exposed to road noise and human voice playbacks to analyse how sound intensity level and duration of both noises altered the time that individuals were active and if their faecal cortisol metabolite (FCM) levels varied. A Hierarchical Analysis Cluster was performed to established two mink groups with respect to both noise source type: short duration/low intensity (SL) and long duration/high intensity (LH). We performed General Linear Mixed Models to evaluate the variation in locomotor activity duration (s) and FCM (ng/g) levels, respectively. The results showed both road noise and human voices decreased locomotor activity duration in SL more sharply compared to LH, and human voices were the triggers that induced the most pronounced response to both exposure conditions. FCM levels increased in SL compared to LH during road noise while the opposite happened during human voices. Differences based on sex and age of individuals were observed. In conclusion, noise characteristics given by the sound type determined the variations in locomotor activity duration while noise exposure level determined the variations in FCM levels. Attention should be paid to noisy activities (e.g. recreational activities for visitors in protected natural areas) and loud groups of people to conserve wildlife, especially noise sensitive species.