Efecto genotóxico del plaguicida fipronil en alevinos de "Gamitana" Colossoma macropomum en condiciones de laboratorio Pesticide genotoxic effect of fipronil in alevins "Gamitana" Colossoma macropomum under laboratory conditions Resumen El presente trabajo evalúa la existencia de daño genotóxico en "Gamitana" ( Colossoma macropomum") al ser expuesta al plaguicida fipronil, para lo cual se utilizó el test de micronucleos (MN). Se trabajó con alevinos, dispuestos en peceras de vidrio, los cuales fueron expuestos a 3 diferentes concentraciones de fipronil (C1: 0.075mg/L, C2: 0.15mg/L y C3: 0.30 mg/L), evaluándose a las 24 y 48 h. Se utilizó sangre periférica para realizar el frotis. El recuento de micronúcleos y de anormalidades se realizó sobre la base de 1000 células. El índice promedio de micronúcleos indica que a 0.075 mg/L de fipronil la frecuencia promedio es el doble del control. Con respecto a las anormalidades del núcleo, se encontró una mayor frecuencia a las 48 h de exposición. Palabras clave: Micronúcleos, daño nuclear, Gamitana, plaguicida.
AbstractThis study evaluates the genotoxic injury in the species Colossoma macropomum ("Gamitana") when exposed to the pesticide Fipronil, for which we used the micronucleus test (MN). We workwed with juveniles in the third stage, placed in glass tanks, which were exposed to 3 different concentrations of fipronil (C1: 0.075 mg /L, C2: 0.15 mg /L and C3: 0.30 mg /L), evaluated at 24 and 48 h. Peripheral blood was used which made the spread. The counting of micronuclei and abnormalities was made on the basis of 1000 cells. The average rate of micronuclei indicates that 0.075 mg / L the average frequency is twice the control. With regard to abnormalities of the core, was found more frequently at 48 h of exposure.
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