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Langmuir films of globulin 11S protein, l-dipalmitoylphosphatidylcholine (L-DPPC), and mixtures of both on water and on buffer subphases were studied. Brewster angle microscopy (BAM) was used to characterize in situ the films morphology along Pi-A isotherms at the air/liquid interface. The L-DPPC monolayer on water behaved as has been reported extensively in the literature but a slight increase on surface pressure and a notable change in domain morphology is observed on buffer. This difference in domain behavior is due to the stabilization interaction of the LE phase by the buffer ions. On the other hand, the protein monolayer was prepared by direct deposit or injection below the surface. Both methods formed mostly a condensed film, with a multilayer formed by globular aggregates in the first method with the two subphases. However, the second method showed different behavior of the protein films depending on the subphase; on water the protein formed a homogeneous film with some globule aggregates, but on buffer a remarkably well-organized monolayer was observed by atomic force microscopy (AFM). Mixtures of globulin 11S and L-DPPC were prepared using both methods for the protein film formation at the air/fluid interface. BAM showed that the mixtures formed coexistence regions between two condensed phases, whose domains of both phases behave like liquids. Fingering phenomena were observed at the interface between protein-rich and L-DPPC-rich domains, which indicates that both phases are fluid. AFM images of the mixtures show the formation of protein- or L-DPPC-rich domains. The liquidlike behavior could be explained due to different sizes of the protein and the L-DPPC, the minority compound in each kind of domain produces defects making them behave as liquids. Interestingly enough, as the monolayer is compressed to higher surface pressure, the lipid molecules are squeezed out and complete separation of the protein and L-DPPC is produced. Furthermore, we present evidence that the protein/L-DPPC mixtures produce films with holes, which might indicate its tendency to form hollow aggregates that could have some relevance in water-channel formation for in vivo seed germination.