ResumenCedrus atlantica es una planta ornamental utilizada para la decoración de parques y jardines. Con el fin de micropropagar material de calidad de esta planta, se implementó un protocolo de multiplicación in vitro. Para ello, como explantes se usaron las yemas axilares que se desinfestaron con hipoclorito de sodio al 10% por 15 min, tratamiento con lo que se obtuvo un 83% de material aséptico. El cultivo de los explantes se realizó en el medio basal McCown's Woody Plant (WPM) adicionado con carbón activado (1 g L -1) y cisteína (25 mg L -1 ), lo que permitió obtener cultivos sin rasgos de oxidación. En la etapa de multiplicación con el uso de la bencil amino purina (5 mg L -1 ) se obtuvieron hasta 5 brotes por explante, que presentaron un crecimiento de 1.4 cm. Sin embargo, el ácido indol butírico a 2.5 y 5 mg L -1 no indujo la formación de raíces en los brotes in vitro.Palabras clave: cedro, brotación, micropropagación, plantas leñosas. IntroducciónCedrus atlantica es una especie arbórea perteneciente a la familia Pinacea conocido como el verdadero cedro. La planta se introdujo a México como una planta ornamental. AbstractCedrus atlantica is an ornamental plant used to decorate parks and gardens. In order to micropropagate quality material of this plant, an in vitro multiplication protocol was implemented. For this, the axillary buds were used as explants which were disinfected with sodium hypochlorite 10% for 15 min, treatment with which obtained 83% aseptic material. Explants culture was performed in the basal medium McCown's Woody Plant (WPM) added with activated carbon (1g L -1 ) and cysteine (25 mg L -1 ), which allowed to obtain crops without oxidation features. In the multiplication stage with the use of benzyl amino purine (5 mg L -1 ) obtained up to 5 shoots per explant, which showed an increase of 1.4 cm. However, indole butyric acid at 2.5 and 5 mg L -1 did not induce root formation in vitro shoots.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.