In Argentina, Eleusine tristachya has been recently reported as a problematic weed that can occur at high densities in spring and summer in fallows and in maize and soyabean. The reason for the increase in E. tristachya populations is that once the weed is established, it is difficult to eliminate because it produces a high number of seeds and plant regrowth occurs after herbicide treatments. The aim of this study was to determine the effect of post‐emergence application of herbicides (glyphosate, haloxyfop‐methyl and clethodim) at the seedling, vegetative and reproductive stages on the short‐term (biomass 30 days after treatment—30 DAT) and long‐term (tiller number and height and seed production—regrowth at 330 DAT) control of E. tristachya selected biotypes in outdoor pot experiments. Data fitted to a log‐logistic model. For all the herbicides considered, at the seedling and vegetative stages, short‐term control was achieved with low ED50 and ED90 values, whereas at the reproductive stage, ED90 values were three‐ to sixfold (glyphosate), six‐ to 52‐fold (haloxyfop‐methyl) and five‐ to 13‐fold (clethodim) higher. Long‐term control at the recommended dose or lower was not possible at the reproductive stage as tiller regrowth and seed production occurred with all herbicides. It is advisable to control Eleusine tristachya when plants are small at the beginning of the growing season using the recommended herbicide dose. A delayed application will produce tiller regeneration the following year, and consequently, control would only be achieved applying an overdose, which can cause risks to health and the environment. We concluded that a management programme based on the combination of glyphosate with post‐emergence graminicides applied at early stages will be effective to control future infestations.