A Bradyrhizobium japonicum USDA 110-derived strain able to produce wider halos in soft-agar medium than its parental strain was obtained by recurrent selection. It was more chemotactic than the wild type towards mannitol and three amino acids. When cultured in minimal medium with mannitol as a single carbon-source, it had one thick subpolar flagellum as the wild type, plus several other flagella that were thinner and sinusoidal. Root adsorption and infectivity in liquid media were 50-100% higher for the selected strain, but root colonization in water-unsaturated vermiculite was similar to the wild type. A field experiment was then carried out in a soil with a naturalized population of 1.8 x 10(5) soybean-nodulating rhizobia g of soil(-1). Bradyrhizobium japonicum strains were inoculated either on the soybean seeds or in the sowing furrows. Nodule occupation was doubled when the strains were inoculated in the sowing furrows with respect to seed inoculation (significant with P<0.05). On comparing strains, nodule occupation with seed inoculation was 6% or 10% for the wild type or selected strains, respectively, without a statistically significant difference, while when inoculated in the sowing furrows, nodule occupation increased to 12% and 22%, respectively (differences significant with P<0.05).

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Th e issue of competition for nodulation has received much attention in studies on soybean [Glycine max (L.) Merr.] crops because native soil rhizobia oft en preclude nodulation of inoculated high-quality strains. In this work, soil placement and enhanced motility of Bradyrhizobium japonicum were investigated as strategies to improve the competitiveness of soybean inoculants applied in the presence of large populations of native (or indigenous) soybean-nodulating rhizobia. Th e fi rst strategy might allow for the enhanced distribution of inoculant rhizobia in the surface soil, compared with inoculant that is directly applied to soybean seed, while the second strategy is expected to enhance root colonization. Th e eff ects of soil placement and enhanced bacterial motility on percentage nodule occupation, grain yield, and grain N contents were examined during the 2004-2005 soybean season at three locations in Argentina, comprising soils classifi ed as entic hapludol, argillic pelludert, and typical argiudol. Th e experimental design was a randomized complete block design, and main eff ects and interactions were also analyzed with a factorial design. Strain LP 3008, previously selected for increased motility, was more eff ective than the commonly inoculated strain E 109. While E 109 occupied, on average, 13.2% of nodules with seed inoculation, LP 3008 occupied 28.2%. In comparison, in-furrow inoculation yielded 24.2 and 37.2% average nodule occupancies for E 109 and LP 3008, respectively. However, these gains in nodulation effi cacy lead to a modest, nonsignifi cant yield increase, and grain N content was unaff ected.

We present the complete genome sequence of Azospirillum brasilense Az39, isolated from wheat roots in the central region of Argentina and used as inoculant in extensive and intensive agriculture during the last four decades. The genome consists of 7.39 Mb, distributed in six replicons: one chromosome, three chromids, and two plasmids.