Stress is present in our everyday lives and it is considered to be the driving force of evolution. Anxiety, chronic worries and panic attacks are the most common consequences of long-term and exhausting stress. The most significant fundamental contribution that is crucial for the development of the modern concept of stress was made by Claude Bernard who proposed the theory that the body was constantly maintaining a stable and well-balanced internal environment or "milieu interieur". Maintaining the internal environment constant was defined as homeostasis by Cannon who proposed two maintenance mechanisms - through negative feedback from the autonomic nervous system and through sensory organs. Hans Selye proposed stress as non-specific strain on the body, caused by an altered body function which is followed by the release of stress hormones. He named this process the general adaptation syndrome which had three stages: alarm reaction, initial phase exhibiting "fight or flight" response; resistance, when body is getting used to being stressed, and exhaustion, when resistance to stress is gradually decreased and collapses. Hans Selye was the first to coin the term "heterostasis" representing the procedure by which a new stable state was achieved by the activation of physiological adaptive mechanisms. Lazarus highlighted emotions as an important factor in behavior in response to stress and provided a description of various reactions to stressors. He emphasized the process of cognitive assessment as a mediator in dealing with stressors - how a person imagines or evaluates an event in order to understand stress reactions in people. Eustress, considered to be positive stress leads to toned emotions, motivation and focused energy, while distress, negative stress, occurs after prolonged stress that exceeds our ability to deal with it. Distress causes anxiety or withdrawal (depression and anxiety), and is accompanied by unpleasant feelings and reduced work ability leading to mental and physical illnesses.

Objective: Mutation of p53 is detected in more than 50% of human cancers, expression of p53 has a potential prognostic value in patients with renal cell carcinoma (RCC). Survivin is a member of the inhibitor of apoptosis protein family, its overexpression is observed in many malignancies, including RCC. The aim of the study was to estimate a correlation between survivin and p53 expression in tumor samples and the histologic type of a tumor, tumor stage, tumor grade, and survival of patients. Materials and Methods: Tumor samples were collected from surgical specimens of 90 patients who underwent radical or partial nephrectomy for RCC between November 2017 and July 2020. Tumors were staged according to the UICC (The Union for International Cancer Control) TNM classification system and histopathologically graded according to Fuhrman nuclear grade system. Histopathological diagnosis was confirmed with standard light microscopic evaluation, using hematoxylin and eosin staining and standard p53 and survivin antibodies. Results: Positive p53 staining was observed in 36.7% of tumor specimens and 24.4% were survivin positive. There was a statistically significant correlation between p53 or survivin expression and histologic subtype of clear cell RCC as well as Type I and II of papillary RCC. There was a statistically significant correlation between p53 expression and tumor size, stage, and grade. The p53 or survivin expression was related to lower overall survival. Conclusion: The results of this study suggest that p53 overexpression and survivin positivity in RCC patients could be associated with poor prognosis. Thus, these proteins could be used as prognostic markers in RCC.

Introduction: Transrectal ultrasound-guided prostate biopsy (TRUS-biopsy) is the "gold standard" in the diagnosis of prostate cancer (PC). There is much divided opinion on the need for biopsy in patients with prostate-specific antigen (PSA) between 4 and 10 ng/ml. The positive biopsy outcome (PC) in these patients ranges from 20 to 39%. Low sensitivity and specificity of PSA in predicting positive biopsy outcome results in a large number of unnecessary biopsies and treatments. In order to better select candidates for biopsy, several risk stratification models for PC have been proposed in recent years, among them the PAMD score. Aim: The aim of this study was to examine the value of the PAMD score in the assessment of positive biopsy outcomes in our population of patients, as well as to examine individual risk factors for PC in patients with PSA values between 4 and 10 ng/ml treated in Serbia. Material and methods: The study involved 50 patients at the Clinic of Urology, University Clinical Centre of Serbia, whose PSA value were measured in the range from 4 to 10 ng/ml. In all the patients we measured PSA and %fPSA, and performed DRE, as well as magnetic resonance imaging (MRI) to evaluate prostate volume (PV) and PI-RADS score. All patients underwent TRUS-guided systemic prostate biopsy. In accordance with the data from literature, PAMD score was determined for all the patients. Results: A PAMD score > 3 showed a high specificity in the prediction of PC, as well as an association with a higher frequency of highgrade PC. A positive finding on DRE, %fPSA< 16, age above 69 years and PI-RADS > 3 showed a statistically significant association with the existence of PC. A high individual predictive value in assessing the presence of PC was confirmed for DRE, %fPSA, PV, and PI-RADS score. Conclusion: The PAMD scoring system may be of importance for better selection of candidates for TRUS-biopsy, in the population of patients with PSA values 4-10 ng/ml.

Introduction: Chronic prostatitis/chronic pelvic pain syndrome (CP/ CPPS) is a inflammatory syndrome, manifested by pain, voiding symptoms, sexual dysfunction, and mental health issues including anxiety. Beneficial effects of exercise are known, but its influence on CP/CPPS has not been investigated. This study aimed to determine the effects of chronic aerobic physical activity on anxiety-related behavior and pain in rats with experimental CP/CPPS. Material and Methods: Adult male Wistar albino rats (n=32) were randomly assigned to 4 groups (n=8 in each): Sham-SED (30-days sedentary-SED protocol on treadmill and intraprostatic injection of 0.9% NaCl); Sham-PA (30-days physically active-PA protocol on treadmill and intraprostatic injection of 0.9% NaCl); CP/CPPS-SED (30-days SED protocol on treadmill and intraprostatic injection of 3% l-carrageenan); CP/CPPSPA (30-days PA protocol on treadmill and intraprostatic injection of 3% l-carrageenan). To establish pain dynamics, scrotal skin pain thresholds were measured by electronic von Frey aesthesiometer (evF) preoperatively: 2 and 1 day, and also postoperatively: 2nd, 3rd, and 7th day. Anxiety-like behavior was estimated by subjectiong the animals to the open field (OF), elevated plus maze (EPM) and light/dark (L/D) tests at the same postoperative days as evF. Results: Rats that developed experimental CP/CPPS showed decreased mechanical pain threshold in the scrotal skin in all postoperative time points, in comparison to the Sham group. Also, in rats with prostatitis increased anxiety-like behavior was observed in OF, EMP and L/D test, compared to corresponding controls. Protocol of 30-day long exercise in rats with CP/CPPS led to reversion of anxiety-like behavior and increased scrotal pain threshold. Conclusion: Results of the present study showed that exercise pretreatment on the treadmill for 30 consecutive days led to the amelioration of anxiety-related and pain-related behavior in rats with CP/CPPS.

Introduction: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is one of the most common diseases of genitourinary system in male population. It is frequently accompanied by psychosocial disorders which include anxiety. Despite numerous clinical studies, the mechanisms of this link are still unclear, partly due to the lack of studies on experimental animal models. Aim: The aim of this study was to examine locomotion activity and anxiety in rats, with experimentally induced CP/CPPS, using the open field test. Material and methods: Wistar male rats were randomly distributed into control and experimental group. In experimental group, CP/CPPS was induced by single intraprostatic injection of 3% λ-carrageenan. Development of pelvic pain syndrome was assessed by evaluating mechanical pain thresholds by electronic Von Frey aesthesiometer (eVF). The rats were individually tested in the open field, seventh day following the intraprostatic injection. Results: In animals from the experimental group, distance and time of ambulatory movement in the open field test were significantly decreased, number of rearing reduced, time spent in the center of the open field significantly decreased, while index of thigmotaxis was significantly increased compared to the control animals. Conclusion: Results of this study indicate anxiogenic effect of CP/CPPS in rats.