The results of clinical and orthopedic examination of highly productive loose cows in the agricultural organization of Sverdlovsk region are presented in the article. Due to the high percentage of pathology of the musculoskeletal system in animals, this farm was selected. As a result of the study, violations of veterinary sanitary norms of maintenance and technological violations in animal feeding were found. On average, more than 40.0% of animals recorded clinical signs of pathology of the musculoskeletal system. Biochemical studies of urine and blood serum were performed to determine the metabolic characteristics of the blood of animals with orthopedic pathology. Hypostenuria was established in 41.7% of the examined cows according to the results of a biochemical study of urine. This indicates a lack of the required level of dry matter consumption. In 31.3% of cows, manifestations of metabolic disorders were recorded (a decrease in total protein and albumin, an increase in urea in the blood serum). In 25.0% of animals revealed the presence of intoxication syndrome - an increase in the activity of indicator liver enzymes (aspartate aminotransferase and glutamate dehydrogenase). Signs of development of osteodystrophic processes (a decrease in total calcium with an increase in alkaline phosphatase activity) were noted in more than 30.0% of cows.