No abstract

Zirconium oxide is a material commonly used in dental prosthetics for making cups of permanent prosthetic restorations. In order to properly prepare the surface of zirconium oxide for prosthetic treatment, it must be veneered with ceramics. The quality of cup-veneered ceramics is dependent on many factors, including the surface free energy (SFE) and transformation of zirconium oxide. The aim of the study was to investigate the type of phase transition and the value of free energy of the surface subjected to machining (wet and dry grinding, polishing). Quantitative and qualitative phase identification measurements showed that mechanical treatment causes transformation of the tetragonal phase into a monoclinic phase in the zirconium oxide surface. Prepared samples were analyzed by means of X-ray diffraction (XRD), which confirmed the phenomenon of transition. Measurements of the wetting angle and the calculated values of the surface free energy (SFE) showed no significant differences between the samples subjected to each treatment

Bacteriophage therapy has emerged as a strategy supplementing traditional disinfection protocols to fight biofilms. The aim of the study was to isolate the phages against E. faecalis and to characterize its biological features, morphology, and lytic activity in a formed biofilm model. Methods: E. faecalis ATCC 29212 strain was used for the trial. Two novel vB_Efa29212_2e and vB_Efa29212_3e virulent phages were isolated from urban wastewater and characterized. The E. faecalis biofilm was established in 15 bovine teeth for 21 days. Transmission (TEM) and scanning electron (SEM) microscopes with the colony-forming unit (CFU) counting were used for assessment. Results: Isolated phages differed in morphology. Taxonomy for vB_Efa29212_2e (Siphoviridae, Efquatovirus) and for vB_Efa29212_3e (Herelleviridae, Kochikohdavirus) was confirmed. Both phages were stable at a temperature range of 4–50 °C and showed a different tolerance to chemicals: 15% EDTA, 1-3% sodium hypochlorite, and chlorhexidine. SEM analysis showed distortion of bacteria cells after phage inoculation, which proved the lytic activity against E. faecalis. A 54.6% reduction in the E. faecalis biofilm confirmed bacteriophage efficacy against isolates in the ex vivo model. Conclusions: Results strongly support the concept that phage therapy has a real therapeutic potential for the prevention and treatment of E. faecalis-associated infections.

Purpose: of this paper was to analyse clinicians’ views on the management and handling procedures of the Biodentine tricalcium silicate cement with the following evaluation of the real setting time of the material with two independent physical tests. Design/methodology/approach: A survey study included 174 clinicians who answered the questionnaire designed to collect opinions on the Biodentine management during endodontic procedures. To verify the setting time of the cement, two independent hardness tests were performed. Macroscopic evaluation was carried out using the Vicat device. Microscopic assessment with subsequent SEM observation was conducted with the aid of the Clemex appliance. Findings: 43% of respondents using Biodentine in their practice described the setting time as long or definitively too long. One fifth of the dentists surveyed continue dental procedures without waiting. The setting time tests confirmed the existence of two phases of the Biodentine setting process, which corresponds to the general definition of cement setting. After mixing of the material, the initial setting stage lasts for 15 minutes. The next one, described by the authors as “maturation” of Biodentine lasts for 120 minutes. Research limitations/implications: The material initially sets within 15 minutes, however it is not the end of the process. In certain endodontic procedures the awareness of a longer setting time of Biodentine is essential for decision-making in root canal therapy. Practical implications: It is advisable to divide the endodontic treatment with Biodentine into two separate appointments. Originality/value: From the clinicians’ perspective the setting time and correct handling of Biodentine are crucial factors in the successful endodontic therapy. The information regarding proper material management is included in this paper.

Background and objectives: Pulp stones are hard tissue structures formed in the pulp of permanent and deciduous teeth. Few studies have evaluated their morphology and chemical composition. However, their formation, composition, configuration and role played in overall health status are still unclear. Clinically, they may be symptomatic; technically, they impede access during endodontic therapy, increasing the risk of treatment errors. Thus, this study aimed to morphologically analyze pulp stones and present their chemical quantification, identifying their main chemical elements. It also correlates the results with their possible induction mechanisms. Materials and Methods: Seven pulp nodules were collected from molar teeth needing endodontic treatment. The morphology of the stones was analyzed by scanning electron microscopy (SEM), and their chemical composition was determined by X-ray dispersive energy spectroscopy (EDX). Results: These structures varied considerably in shape, size and topography. The site of the stones in the pulp cavity was the factor that most affected the morphology. The majority of the stones found in the pulp chambers presented nodular morphology, while those in the root canals presented a diffuse shape, resembling root canal anatomy. The topography of the nodules showed heterogeneous relief, revealing smooth and compact areas contrasting with the rugged and porous ones. The chemical composition varied depending on the location of the nodule in the pulp cavity and the relief of the analyzed area. Radicular stones presented considerably lower calcium and phosphorus content than coronary nodules. Conclusions: The high cellularity rate of the coronal pulp predisposes this region to nodular mineralizations around injured cells. The presence of larger caliber vascular bundles and higher collagen fiber content in radicular pulp determines a diffuse morphological pattern in this region. Understanding the morphology and chemical composition of the pulp stones allows future translational pathways towards the prevention or treatment of such conditions.