The protein ataxin-3 consists of an N-terminal globular Josephin domain (JD) and an unstructured C-terminal region containing a stretch of consecutive glutamines that triggers the neurodegenerative disorder spinocerebellar ataxia type 3, when it is expanded beyond a critical threshold. The disease results from misfolding and aggregation, although the pathway and structure of the aggregation intermediates are not fully understood. In order to provide insight into the mechanism of the process, we monitored the aggregation of a normal (AT3Q24) ataxin-3, an expanded (AT3Q55) ataxin-3, and the JD in isolation. We observed that all of them aggregated, although the latter did so at a much slower rate. Furthermore, the expanded AT3Q55 displayed a substantially different behavior with respect to the two other variants in that at the latest stages of the process it was the only one that did the following: i) lost its reactivity towards an anti-oligomer antibody, ii) generated SDS-insoluble aggregates, iii) gave rise to bundles of elongated fibrils, and iv) displayed two additional bands at 1604 and 1656 cm−1 in FTIR spectroscopy. Although these were previously observed in other aggregated polyglutamine proteins, no one has assigned them unambiguously, yet. By H/D exchange experiments we show for the first time that they can be ascribed to glutamine side-chain hydrogen bonding, which is therefore the hallmark of irreversibly SDS-insoluble aggregated protein. FTIR spectra also showed that main-chain intermolecular hydrogen bonding preceded that of glutamine side-chains, which suggests that the former favors the latter by reorganizing backbone geometry.
Protein misfolding and aggregation in intracellular and extracellular spaces is regarded as a main marker of the presence of degenerative disorders such as amyloidoses. To elucidate the mechanisms of protein misfolding, the interaction of proteins with inorganic surfaces is of particular relevance, since surfaces displaying different wettability properties may represent model systems of the cell membrane. Here, we unveil the role of surface hydrophobicity/hydrophilicity in the misfolding of the Josephin domain (JD), a globular-shaped domain of ataxin-3, the protein responsible for the spinocerebellar ataxia type 3. By means of a combined experimental and theoretical approach based on atomic force microscopy, Fourier transform infrared spectroscopy and molecular dynamics simulations, we reveal changes in JD morphology and secondary structure elicited by the interaction with the hydrophobic gold substrate, but not by the hydrophilic mica. Our results demonstrate that the interaction with the gold surface triggers misfolding of the JD when it is in native-like configuration, while no structural modification is observed after the protein has undergone oligomerization. This raises the possibility that biological membranes would be unable to affect amyloid oligomeric structures and toxicity.
Using nCB films adsorbed on MoS 2 substrates studied by x-ray diffraction, optical microscopy and Scanning Tunneling Microscopy, we demonstrate that ordered interfaces with well-defined orientations of adsorbed dipoles induce planar anchoring locked along the adsorbed dipoles or the alkyl chains, which play the role of easy axes. For two alternating orientations of the adsorbed dipoles or dipoles and alkyl chains, bi-stability of anchoring can be obtained. The results are explained using the introduction of fourth order terms in the phenomenological anchoring potential, leading to the demonstration of first order anchoring transition in these systems. Using this phenomenological anchoring potential, we finally show how the nature of anchoring in presence of dual easy axes (inducing bi-stability or average orientation between the two easy axes) can be related to the microscopical nature of the interface. IntroductionUnderstanding the interactions between liquid crystal (LC) and a solid substrate is of clear applied interest, the vast majority of LC displays relying on control of interfaces. However this concerns also fundamental problems like wetting phenomena and all phenomena of orientation of soft matter bulk induced by the presence of an interface. In LCs at interfaces, the so-called easy axes correspond to the favoured orientations of the LC director close to the interface. If one easy axis only is defined for one given interface, the bulk director orients along or close to this axis [1]. It is well known that, in anchoring phenomena, two major effects compete to impose the anchoring directions of a liquid crystal, first, the interactions between molecules and the interface, second, the substrate roughness whose role has been analyzed by Berreman [2]. The influence of adsorbed molecular functional groups at the interface is most often dominant with, for example in carbon substrates, a main influence of unsaturated carbon bonds orientation at the interface [3]. In common LC displays, there is one unique easy axis, but modifications of surfaces have allowed for the discovery of promising new anchoring-related properties. For instance, the first anchoring bistability has been established on rough surfaces, associated with electric ordo-polarization [4] and the competition between a stabilizing short-range term and a destabilizing long-range term induced by an external field, can induce a continuous variation of anchoring orientation [5]. More recently, surfaces with several easy axes have been studied extensively. It has been shown that control of a continuous variation of director pretilt, obtained in several systems [6,7], is associated with the presence of two different easy axes, one perpendicular to the substrate (homeotropic) and one planar [7,8]. Similar models can explain the continuous evolution of anchoring between two planar orientations observed on some crystalline substrates [9]. However, in the same time, two easy axes can also lead to anchoring bi-stability [10,11] or discontinuous transi...
Amelogenin refers to a class of intrinsically disordered proteins that are the major constituents of enamel matrix derivative (EMD), an extract of porcine fetal teeth used in regenerative periodontal therapy. Modifications in molecular conformation induced by external stresses, such as changes in temperature or pH, are known to reduce the effectiveness of EMD. However, detailed descriptions of the conformational behavior of native amelogenin are lacking in the open literature. In the present work, a molecular model for the secondary and tertiary structure of the full-length major porcine amelogenin P173 was constructed from its primary sequence by replica exchange molecular dynamics (REMD) simulations. The REMD results for isolated amelogenin molecules at different temperatures were shown to be consistent with the available spectroscopic data. They therefore represent an important first step toward the simulation of the intra- and intermolecular interactions that mediate self-organization in amelogenin and its behavior in the presence of other EMD components under conditions representative of its therapeutic application.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.