This study investigated the migratory fish spawning within the reservoirs of the Machadinho and Itá dams (upper Uruguay River, Brazil) and its relationship to environmental variables. Sampling was conducted in the lotic region of the river in two sites between the dams' reservoirs: Uruguay (main river) and Ligeiro (tributary). Sampling included nine consecutive reproductive periods (RP) spanning the period from 2001 to 2010 and was conducted at night on the water surface using cylindrical-conical plankton nets (0.5 mm mesh); environmental variables were also recorded. The spawning of the migratory species Salminus brasiliensis, Prochilodus lineatus, and Steindachneridion scriptum was registered: S. brasiliensis and P. lineatus spawned in the tributary river at the end of spring/beginning of summer, during flooding and during periods of high water temperature. Steindachneridion scriptum spawned in the main river at the beginning of spring. The study showed that S. brasiliensis, P. lineatus, and S. scriptum are able to spawn in small lotic river stretches within two reservoirs, but only under very specific and not common environmental conditions. Este estudo verificou a presença de desova de peixes migradores entre os reservatórios das Usinas Hidrelétricas de Machadinho e Itá (alto rio Uruguai, Brasil), e sua relação com as variáveis ambientais. As amostragens foram realizadas na região lótica do rio Uruguai, no trecho situado entre os reservatórios de Itá e Machadinho, em duas estações de amostragem: Uruguai (Principal) e Ligeiro (Tributário). As coletas abrangeram nove períodos reprodutivos (PR) consecutivos, desde 2001 até 2010, e foram realizadas no período noturno, na superfície, com o uso de redes de plâncton do tipo cilíndrico-cônicas de malha 0,5 mm, quando também foram registradas as variáveis ambientais. O estudo registrou a desova de três espécies migradoras: Salminus brasiliensis, Prochilodus lineatus e Steindachneridion scriptum. As populações de S. brasiliensis e P. lineatus desovaram no rio tributário no final da primavera e início do verão em condições de enchentes e temperatura da água mais alta. Steindachneridion scriptum desovou no rio principal durante o início da primavera. O presente estudo mostra que S. brasiliensis, P. lineatus e S. scriptum conseguem desovar em pequenos trechos lóticos de rio entre dois reservatórios, porém em condições ambientais determinadas e pouco frequentes.
The genetic variability of four fish species (Pimelodus maculatus, Prochilodus lineatus, Salminus brasiliensis and Steindachneridion scripta) collected in the upper Uruguay River basin was analyzed using the
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