The use of computer-based and online education systems has made new data available that can describe the temporal and process-level progression of learning. To date, machine learning research has not considered the impacts of these properties on the machine learning prediction task in educational settings. Machine learning algorithms may have applications in supporting targeted intervention approaches. The goals of this paper are to: 1) determine the impact of process-level information on machine learning prediction results and 2) establish the effect of type of machine learning algorithm used on prediction results. Data were collected from a university level course in human factors engineering (n = 35), which included both traditional classroom assessment and computer-based assessment methods. A set of common regression and classification algorithms were applied to the data to predict final course score. The overall prediction accuracy as well as the chronological progression of prediction accuracy was analyzed for each algorithm. Simple machine learning algorithms (linear regression, logistic regression) had comparable performance with more complex methods (support vector machines, artificial neural networks). Process-level information was not useful in post-hoc predictions, but contributed significantly to allowing for accurate predictions to be made earlier in the course. Process level information provides useful prediction features for development of targeted intervention techniques, as it allows more accurate predictions to be made earlier in the course. For small course data sets, the prediction accuracy and simplicity of linear regression and logistic regression make these methods preferable to more complex algorithms.

In recent years, a surge of interest in "flying cars" for city commutes has led to rapid development of new technologies to help make them and similar on-demand mobility platforms a reality. To this end, this paper provides analyses of the stakeholders involved, their proposed operational concepts, and the hazards and regulations that must be addressed. Three system architectures emerged from the analyses, ranging from conventional air taxi to revolutionary fully autonomous aircraft operations, each with vehicle safety functions allocated differently between humans and machines. Advancements for enabling technologies such as distributed electric propulsion and artificial intelligence have had major investments and initial experimental success, but may be some years away from being deployed for on-demand passenger air transportation at scale.

Procedures were developed to maximize the yield of high-quality RNA from small amounts of plant biomass for microarrays. Two disruption techniques (bead milling and pestle and mortar) were compared for the yield and the quality of RNA extracted from 1-week-old Arabidopsis thaliana seedlings (approximately 0.5-30 mg total biomass). The pestle and mortar method of extraction showed enhanced RNA quality at the smaller biomass samples compared with the bead milling technique, although the quality in the bead milling could be improved with additional cooling steps. The RNA extracted from the pestle and mortar technique was further tested to determine if the small quantity of RNA (500 ng-7 microg) was appropriate for microarray analyses. A new method of low-quantity RNA labeling for microarrays (NuGEN Technologies, Inc.) was used on five 7-day-old seedlings (approximately 2.5 mg fresh weight total) of Arabidopsis that were grown in the dark and exposed to 1 h of red light or continued dark. Microarray analyses were performed on a small plant sample (five seedlings; approximately 2.5 mg) using these methods and compared with extractions performed with larger biomass samples (approximately 500 roots). Many well-known light-regulated genes between the small plant samples and the larger biomass samples overlapped in expression changes, and the relative expression levels of selected genes were confirmed with quantitative real-time polymerase chain reaction, suggesting that these methods can be used for plant experiments where the biomass is extremely limited (i.e. spaceflight studies).

Given the rise of autonomous systems in transportation, medical, and manufacturing industries, there is an increasing need to understand how such systems should be designed to promote effective interactions between one or more humans working in and around these systems. Practitioners often have difficulties in conducting costly and time-consuming human-in-the-loop studies, so an analytical strategy that helps them determine whether their designs are capturing their planned intent is needed. A traditional top-down, hypothesis-driven experiment that examined whether external displays mounted on autonomous cars could effectively communicate with pedestrians led to the conclusion that the displays had no effect on safety. However, by first taking a bottom-up, data-driven machine learning approach, those segments of the population that were most affected by the external displays were identified. Then, a hypothesis-driven, within-subjects analysis of variance revealed that an external display mounted on an autonomous car that provided the vehicle’s speed as opposed to commanding a go/no-go decision provided an additional 4 feet of safety for early adopters. One caveat to this approach is that the selection of a specific algorithm can significantly influence the results and more work is needed to determine the sensitivity of this approach with seemingly similar machine learning classification approaches.