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NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of S-20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization.

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Mouse and rat liver, kidney and mammary gland explants were bombarded with high‐velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT‐cat, pχ‐Cusein‐cat, pβ‐Cusein‐cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT‐CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying a pTAT‐cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.

No abstract