This review describes the European Union and the US regulations applicable to food colours. Despite the different regulatory frameworks, the overall approach is similar, based on wellestablished risk-assessment procedures and risk-management measures. However, differences impacting free movement of goods can be found in the details and implementation of regulations. Using additives approved only in the US or in the EU implies that producers aiming to export need to adjust their product composition to the export market. Failure to comply may give rise to claims of adulteration, misbranding or non-compliance and rejection at the border or recall from the market. A careful comparison of the level of protection provided by the two sets of regulations, the criteria of good manufacturing practice (GMP) inspections and the certification requirements could be key to aligning the rules and to negotiating mutual recognition agreements. This review provides an extensive overview of the similarities and differences in regulating food colours in the EU and the US. ARTICLE HISTORY

The photolytic transformation of decabromodiphenyl ethanea current-use brominated flame retardant and major substitute of the structurally related decabromodiphenyl etherwas investigated in different solvents (toluene, dichloromethane, chlorobenzene, and benzyl alcohol). The transformation rate followed pseudo first order kinetics, with increasing half-life (t 1/2) in the order of toluene (t 1/2 = 4.6 min), chlorobenzene (t 1/2 = 14.0 min), dichloromethane (t 1/2 = 27.9 min), and benzyl alcohol (t 1/2 ≈ 60 min). Formation and amount of transformation products varied depending on the solvent used. A detailed study of the hydrodebromination products allowed us to tentatively assign all three possible nonaBDPEs (BDPE 207, 208, and in benzyl alcohol only BDPE 206) and three predominant octaBDPE congeners (BDPE 197, 201, and 202). Next to the reported BDPEs, formation of several oxygen containing transformation products (OxyTPs), dominated by octabrominated OxyTP, was verified by GC-Orbitrap-HRMS analysis. Use of HPLC and Florisil column enabled the separation of OxyTPs and BDPEs, and the polybrominated OxyTPs were most likely tricyclic compounds with almost planar structure.

UV transformation was studied with three structurally closely related current-use brominated flame retardants (cuBFRs), i.e., hexabromobenzene (HBB), pentabromotoluene (PBT), and pentabromoethylbenzene (PBEB). Irradiation in toluene and benzotrifluoride (BTF) showed pseudo-first-order kinetics. Repeated high-performance liquid chromatographic (HPLC) fractionation, available reference standards, dedicated syntheses, gas chromatography with mass spectrometry (GC/MS), GC separation on two different phases including retention time rules based on dipole interactions, and proton magnetic resonance spectroscopy ( 1 H NMR) evaluation enabled a full structural characterization of all 22 transformation products formed by hydrodebromination. In addition to pentabromobenzene (only transformation product with five bromine), tetra-and tribrominated transformation products were predominantly formed in the case of all three cuBFRs. Hydrodebromination was favored by bromine removal from positions with a high Br density. Br → H exchange was about 3 times faster in positions flanked by two vicinal Br atoms. This favored pathway explained why hydrodebromination sharply dropped at the level of tribrominated cuBFRs because readily degradable precursors were no more available at this point. Hence, a full degradation of tribrominated and lower-brominated transformation products may only be achieved in combination with a different process such as microbial transformation.

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Aims Toxaphene is a persistent organic pollutant, composed of approximately 1000 highly chlorinated bicyclic terpenes. The purpose of this study was to evaluate if camphor, a structural analogue of toxaphene, could stimulate aerobic biotransformation of weathered toxaphene. Methods and Results Two enrichment cultures that degrade camphor as the sole carbon source were established from contaminated soil and biosolids. These cultures were used to evaluate aerobic transformation of weathered toxaphene. Only the biosolids culture could transform compounds of technical toxaphene (CTTs) in the presence of camphor, while no transformation was observed in the presence of glucose or with toxaphene as a sole carbon source. The transformed toxaphene had lower concentration of CTTs with longer retention times, and higher concentration of compounds with lower retention times. Gas chromatography with electron capture negative ion mass spectrometry (GC/ECNI‐MS) showed that aerobic biotransformation mainly occurred with Cl8‐ and Cl9‐CTTs compounds. The patterns of Cl6‐ and Cl7‐CTTs were also simplified albeit to a much lesser extent. Seven camphor‐degrading bacteria were isolated from the enrichment culture but none of them could degrade toxaphene. Conclusion Camphor degrading culture can aerobically transform CCTs via reductive pathway probably by co‐metabolism using camphor as a co‐substrate. Significance and Impact of the Study Since camphor is naturally produced by different plants, this study suggests that stimulation of aerobic transformation of toxaphene may occur in nature. Moreover plants, which produce camphor or similar compounds, might be used in bioremediation of contaminated soils.