PURPOSE:To investigate if the association of fat grafts and platelet-rich plasma (PRP) improves graft viability in female rats. METHODS:This is an experimental, randomized and blinded study, which involved 47 rats. Fat was harvested from the inguinal region and grafted to the cranial region. The experimental group consisted of PRP-enriched fat grafts (n=22) whilst the control group consisted of fat graft only (n=25). After a 100-day period, the animals were euthanised and the fat grafts were analyzed using scores from 0 (absent) to 4 (abundant), in optical microscopy by two independent and blinded pathologists. RESULTS:Regarding fat graft cell viability, the PRP group scored moderate/abundant in 63% of cases and the fat graft only group scored absent/slight in 72% of cases (p=0.03). The PRP group also presented lower fat necrosis scores when compared to the fat graft only group (p=0.03). Tumors (dermoid cysts) within the fat grafts were observed in three animals in which the grafts were mixed with PRP. CONCLUSION:Platelet-rich plasma improves the viability and integration of fat grafts in rats, but more studies are needed to fully understand the exact mechanisms that lead to this improvement and assess the safety of the method for use in humans.

Rev Bras Cir Plást. 2012;27(4):616-22 616 Blumenschein AR et al. Breast fat grafting: experimental or established procedure? Lipoenxertia nas mamas: procedimento consagrado ou experimental?ABSTRACT There has recently been an increase in the use of fat grafting for the correction of congenital or acquired breast deformities and for aesthetic breast treatment. This increase is due to the emergence of novel fat grafting techniques that produce lasting and reliable results despite some authors' beliefs that the procedure might hinder breast cancer screening. This literature review aims to identify the most frequently used breast fat grafting techniques, evaluate their efficacy and safety, and report their major complications. The review was performed after a search in the PubMed and LILACS databases, and only English-language articles published over the past 5 years were assessed. There have been an increased number of studies on the topic in recent years, and a specific fat grafting technique has been primarily reported. Most studies in this area have shown that breast fat grafting does not affect radiological breast cancer screening, whereas some studies have suggested that the use of grafted fat may enhance the development of breast cancer. Despite the increased number of published studies, few have a good level of scientific evidence. This review concludes that fat grafting is an alternative technique for the correction of breast deformities and moderate aesthetic breast enlargement and should be performed by properly trained surgeons accompanied by a radiology team that is experienced in breast imaging. Further studies with appropriate scientific methodologies are needed to evaluate breast fat grafting. Keywords: Breast fat grafting. Fat graft RESUMOAtualmente vem ocorrendo aumento da incidência de utilização de enxertia de gordura para correção de defeitos mamários congênitos ou adquiridos e para tratamento mamário estético. Esse aumento é decorrente do surgimento de novas técnicas de lipoenxertia, que produzem resultados mais duradouros e confiáveis, apesar da crença de alguns autores de que o procedimento possa dificultar o rastreamento do câncer mamário. Esta revisão de literatura tem como objetivo identificar as técnicas de lipoenxertia mamárias mais utilizadas, avaliá-las quanto à eficácia e à segurança, e relatar as principais complicações associadas. A revisão foi realizada nas bases de dados PubMed e LILACS, e os fatores de inclusão foram: artigos em idioma inglês, publicados nos últimos cinco anos. Como resultado observou-se aumento do número de trabalhos abordando o tema nos últimos anos, e predomínio de uma técnica de lipoenxertia específica. A maioria dos trabalhos demonstra que a lipoenxertia mamária não prejudica o rastreamento radiológico para câncer mamário, e alguns sugerem que a gordura enxertada potencializa o desenvolvimento de câncer nas mamas. Apesar do aumento do número de trabalhos, existem ainda poucos com bom nível de evidência científica. Esta revisão permitiu concluir que a lipoe...

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Autologous fat grafts and lipofilling can be used for breast reconstruction after breast conserving therapy. Fat is considered an ideal filler because of its low cost, ease of harvest, abundance in the human body and low immunogenic and allergic reaction due to its autologous nature. Platelet rich plasma (PRP) is a plasma fraction, with platelet count above baseline, generally obtained via centrifugation of blood. PRP theoretically promotes tissue regeneration due to fact that it concentrates a greater amount of growth factors essential in the process of tissue regeneration and neovascularization. This paper aims to examine if the association of fat grafts and PRP improves graft viability in female rats, through an experimental, randomized and blinded study, which involved 47 animals. These animals underwent fat graft harvest from their inguinal fat deposits and fat grafting subcutaneously to their cranial region. In 22 animals the fat graft was mixed with PRP and in 25 the fat was grafted by itself. After a 100 day period, the animals were sacrificed and the fat grafts were analyzed using scores from 0 (absent) to 4 (abundant), in optical microscopy by two independent and blinded pathologists, by means of the following variables: fat graft cell viability, fat necrosis, tissue inflammation and fibrosis. Regarding fat graft cell viability, the PRP group scored moderate/abundant in 63% of the cases and the fat graft only group scored absent/slight in 72% of the cases (p<0.05). The PRP group presented lower fat necrosis scores and lower tissue inflammation scores when compared to the fat graft only group (p<0.05). The presence of tissue fibrosis was rarely observed in both groups. Tumors (dermoid cysts) within the fat grafts were observed in 3 animals in which the grafts were mixed with PRP. It is concluded that PRP improves the viability and integration of fat grafts in rats, but more studies are needed to fully understand the exact mechanisms that lead to this improvement and assess the safety of the method for use in humans. Citation Format: Ruffo Freitas-Junior, Alexandre R Blumenschein, Marise AR Moreira, Maria-Auxiliadora PC Cysneiros, Roseana N Pereira. Is the combination of fat grafts and platelet rich plasma effective and safe? An experimental study in rats [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P2-14-04.