We report the isolation, identification, and assemblies of three antibiotic-producing soil bacteria ( Staphylococcus pasteuri , Peribacillus butanolivorans , and Micrococcus yunnanensis ) that inhibit the growth of Neisseria commensals in coculture. With pathogenic Neisseria strains becoming increasingly resistant to antibiotics, bioprospecting for novel antimicrobials using commensal relatives may facilitate discovery of clinically useful drugs.