Two inclusions of leafy liverworts in Miocene Mexican amber are tentatively assigned to the extant genus Mastigolejeunea (Lejeuneaceae subfam. Ptychanthoideae). Both specimens share complicate bilobed leaves with a rounded lobe apex, a lobule with an oblique free margin continuing into the ventral margin of the leaf lobe, elongate leaf cells, a broad ventral merophyte, and undivided underleaves with a truncate to slightly emarginate, recurved apex. The lack of generative structures prevents a more thorough taxonomic treatment. Nevertheless, the inclusions represent the first ptychanthoid Lejeuneaceae from Mexican amber.