This study was implemented to evaluate the potential of near-infrared reflectance spectroscopy (NIRS) technology to estimate the chemical composition and in vitro digestibility of botanically complex herbage mixtures characterised, moreover, by a noteworthy variation among samples in the maturity of the forage plants. A total of 107 herbage samples harvested from permanent meadows located in the uplands of León (northwestern Spain) were analysed to determine their chemical composition. In addition, the in vitro digestibility of each herbage sample was measured by two different in vitro procedures using buffered rumen fluid. A Bran + Luebbe InfraAlyzer 500 spectrophotometer was used to obtain the near-infrared spectra corresponding to each herbage sample. Prediction equations developed for the estimation of the chemical components showed that NIRS technology could predict these parameters accurately, especially the crude protein and neutral detergent fibre contents (R 2 adj > 0.95 in both cases). In vitro digestibility parameters could also be predicted with an acceptable degree of accuracy using NIRS technology, particularly the in vitro Tilley and Terry organic matter digestibility (R 2 adj = 0.925, standard error of prediction (SEP) = 2.165% organic matter) and the in vitro dry matter true digestibility measured according to the Goering and Van Soest procedure (R 2 adj = 0.891, SEP = 2.208% dry matter).

-The aim of the present study was to investigate the influence of cutting frequency, harvest season and nitrogen fertilization on yield and floristic composition of the herbage harvested from a mountain meadow. The meadow was divided into 22 plots, each receiving a different N P K fertilization treatment. The study lasted seven years, and during the first three years (1985)(1986)(1987) each plot was harvested twice per year (June and September) according to a traditional harvest system, whereas in the years 1989-1991 each plot was harvested three times per year (spring, summer and autumn) following a more intensive harvest system. Nitrogen fertilizer favoured the development of the grasses in spring, thus reducing the proportion of legumes in the two cut harvest system, whereas grasses reached an advanced stage of maturity before the first cut in late June. However, the three cut harvest system entailed an earlier first cut in spring. As a result, the production of legume biomass was significantly increased both in the first cut and in the subsequent regrowths. No significant response in total herbage production to the N fertilizer was observed in the more intensive harvest system. Therefore, the three cut harvest system without nitrogen fertilization seemed to be the most suitable practice for the management of these botanically-complex mountain meadows. These results may contribute to design fertilization and management practices of mountain hay meadows to optimize their productivity and sustainability.Key Words: chemical composition, cutting frequency, floristic composition, herbage yield, nitrogen fertilization Influência da estação da colheita, freqüência de corte e fertilização nitrogenada de prados de montanha sobre o rendimento, a composição botânica e o conteúdo de proteína da forragem RESUMO -O objetivo neste estudo foi investigar a influência da freqüência de corte, da estação de colheita e da adubação nitrogenada no rendimento e na composição botânica da forragem recolhida de um prado de montanha. O prado foi dividido em 22 parcelas, cada uma com nível de fertilização N P K. O estudo durou sete anos e, durante os três primeiros (1985)(1986)(1987), cada parcela foi cortada duas vezes por ano (junho e setembro), seguindo um sistema de aproveitamento tradicional, ao passo que, nos anos 1989-1991, cada parcela foi cortada três vezes por ano (primavera, verão e outono), seguindo um sistema de aproveitamento mais intensivo. O fertilizante nitrogenado favoreceu o desenvolvimento das gramíneas na primavera, reduzindo a proporção de leguminosas no sistema de dois cortes anuais, de modo que as gramíneas alcançaram etapa avançada de maturidade antes do primeiro corte, ao final do mês de junho. No entanto, com o sistema de três cortes anuais, o primeiro corte foi realizado mais cedo, na primavera. Conseqüentemente, a produção da biomassa de leguminosas aumentou significativamente, tanto no primeiro corte como nos rebrotes subseqüentes. Nenhum efeito significativo na produção total de forragem em respost...

One-hundred and ninety-two herbage samples from permanent meadows located in the mountains of León (NW Spain) were analyzed for total nitrogen (total N), nitrogen in trichloroacetic acid precipitated matter (TCAN), borate-phosphate buffer insoluble nitrogen (BPBN), neutral-detergent insoluble nitrogen (NDIN) and acid-detergent insoluble nitrogen (ADIN). These data were used to calculate the partition of nitrogen fractions proposed by the Cornell Net Carbohydrate and Protein System (CNCPS): A (total N − TCAN), B 1 (TCAN − BPBN), B 2 (BPBN − NDIN), B 3 (NDIN − ADIN) and C (ADIN). Near-infrared reflectance spectroscopy (NIRS) technology accurately predicted total N, TCAN and NDIN, as judged by coefficient of determination (R 2 ) and ratio performance deviation (RPD) values greater than 0.90 and 2.5, respectively. The rest of the insoluble N fractions (BPBN and ADIN) were predicted with less accuracy by NIRS. Estimations of CNCPS N fractions (A, B 1 , B 2 , B 3 and C) using visible-NIR spectra of forage samples did not allow accurate predictions (R 2 < 0.90; RPD<2.5).

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Sixty-two herbage samples, harvested in natural meadows located in the mountains of León (north-west Spain), and characterised by a diverse botanical composition and different stages of maturity of the plants, were used to evaluate the ability of chemical composition and near infrared (NIR) spectroscopy to predict in vitro digestibility and in sacco degradability of the neutral detergent fibre (NDF) fraction. In vitro digestibility was performed as described by the Goering and Van Soest procedure. Three dry Holstein-Friesian cows fitted with a rumen cannula were used to incubate the herbage samples. A Bran+Luebbe InfraAlyzer 500 spectrophotometer was used to obtain the NIR spectra corresponding to the 62 original herbage samples. Prediction equations for the estimation of in vitro digestibility and in sacco degradability parameters of the NDF fraction were generated using NIR spectra or chemical data as independent variables. The results showed that the in vitro digestibility and kinetic parameters of degradation of the NDF fraction could not be predicted accurately, probably as a consequence of the errors corresponding to the reference methods. In contrast, these errors did not greatly affect the extent of disappearance of the NDF fraction at later times, so the accuracy of prediction of these parameters was higher, especially when NIR spectra were used as independent variables. This is probably due to the close relationship that the parameters showed with the chemical data, since this kind of information, together with some physical characteristics of the samples, is included in the NIR spectra.