The experiment was conducted in the experimental field of the Horticulture Department, University for Development Studies (UDS), Nyankpala campus with the aim of determining the response of three cucumber cultivars (Marketer, Nandini, Pointset) to the application of sulphate of ammonia as top dressing with respect to fruit yield, and shelf life. It was a 3 x 3 x 2 factorial arranged in a randomized complete block design with three replications. Data was collected on total fruit yield, shelf life and percentage fruit decay in storage. Their effect on the varieties differed significantly to a varying degree. The factors, however, had no significant impact and caused no difference in the fruit yield and fruit weight. 15:15:15 NPK effect on the cucumbers caused a high decay incidence when applied alone or in combination with (NH4)2SO4. Therefore, the application rate may be revised or reduced to experiment if decay incidence would decline in storage. Further studies may extensively be carried out to note if varying the rate of NPK would impact positively on the shelf life of cucumber. (NH4)2SO4 effect increased weight loss in cucumbers hence, better storage techniques should be considered by controlling high temperatures. Complete fertilizer; 15:15:15 NPK and (NH4)2SO4 had a positive impact on protein and fibre. Hence, their application is recommended for increasing protein and fibre level in cucumbers.