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Background: Plasma concentrate products have Rapidly developed during the last few years, and it had significantly improved regenerative medicine because of its specific features which enhance healing and reduce pain after surgeries. this research aims to improve the antibacterial effectiveness of PRF by adding different forms of antibiotics and evaluate the physical and antibacterial properties of the new PRF. Methods: In-Vitro experimental study was conducted; modified protocol was used which use two stages of centrifuging was used prepare PRF. Bacterial colonies and Miller-Hinton medium was prepared. Two types of bacteria were used for the culturing and incubation (Staphylococcus aureus, Enterococcus faecalis). Different forms and volumes of antibacterial agents (Lincomycin) were incorporated with the PRF. Physical and antibacterial properties were evaluated.Results: ampoule and solution forms had the highest levels of bacterial growth inhibiting, and best physical properties, bacterial inhibiting lasted for 10 days in culture. Significant difference was noticed between the modified PRF and the control group (p<0.05). adding (0.5 ml) of ampoule form had the best results on both antibacterial and physical properties of the new PRF. Conclusion: The protocol proposed in this study succeeded to improve the antibacterial activity of the PRF with preserving the physical properties. No significant difference in physical properties were noticed between control group and PRF with antibiotic. Significance and impact of the study: PRF can be used as a local antibiotic delivering biomaterial adjacent to minor surgery, the results of this study can be used to reduce the post-operative infection that is frequent after oral surgeries.

Background: Plasma concentrate products have Rapidly developed during the last few years, and it had significantly affected regenerative medicine because of its specific features which enhance healing and reduce pain after surgeries. this research aims to create an antibiotic releasing biomaterial based on the PRF.Methods: In-Vitro experimental study was conducted, 12 Healthy volunteers were included, each donated (40 ml) of blood. Chukrun’s protocol was used to prepare PRF (3000 rpm, 10m) with additional modification. Bacterial colonies and Miller-Hinton medium was prepared. Two types of bacteria were used for the culturing and incubation (Staphylococcus aureus, Enterococcus faecalis). Three Pharmaceutical forms (PF) of the antibacterial agents (Lincomycin) were incorporated with the PRF. Results: pharmaceutical form had a significant difference on the antibacterial activity of the PRF (P=0.000), ampoule and solution forms had the highest levels of bacterial growth inhibiting, however, powder failed to inhibit bacterial growth. Moreover, no significant difference was noticed between using 0.5ml or 1ml dose of antibiotic (P=339). Conclusion: It is possible to incorporate antibiotic with the PRF, which will improve the antibacterial activity of PRF, creating an ARB that can be used locally adjacent to minor surgery.

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