The experiment was carried out in the laboratory of tissue culture of Plant Production Techniques Department, at Musaib Technical College to study the effect of different concentrations of putrescine and the type of light on the content of the Gardenia from some effective medical compounds. The experiment included two factor the first consisted of two sources of light, namely light (fluorescent), LED light (18 red: 2 blue) and the second factor, the addition of Putrescine with three concentrations (0.5, 1 and 1.5) mg.l-1. Some effective medical substances (Coumaric, Ferulic, Caffeorylquanic, Sinapic acid and Tannic) acids was measured in Callus Of Gardenia using high-performance liquid chromatography (HPLC).The results were analyzed using the statistical program Genstat and the mean was measured according to the least significant difference of LSD at the probability level of 5%. The results showed superior treatment of LED lighting in the concentration of all measured active compounds, The highest concentration of compounds (Ferulic, Caffeorylquanic and Sinapic) acids was given with a concentration of 0.5 mg.l-1. The highest concentration of compounds (Coumaric and Tannic) acids. The interaction (LED + Putrescine at 0.5) gave the highest concentration of compounds (Ferulic, Caffeorylquanic and Sinapic) in Callus of Gardenia.

Researchers from the College of Agriculture at the University of Kerbala in the Karbala province of Iraq used a greenhouse to examine the effects of nano-potassium and the amino acids tryptophan and glycine on the development of the stevia plant and the concentration of its active substances in the local climate. The experiment had three replicates and was carried out using a factorial trial design within a randomized complete block design (R.C.B.D). The study had three variables. The first component was tryptophan, with three concentrations (0, 25, and 50 mg. L-1), the second factor was glycine, with three concentrations (0, 500, and 1000 mg. L-1), and the third factor was nano-potassium, with two values (0, and 100 nmol L-1) (0, 2 g. L-1). The findings demonstrated that the variables under study, whether acting separately or in combination, significantly improved all characteristics of vegetative development. The triple interaction treatment (50 mg. L-1 tryptophan + 1000 mg. L-1 glycine + 2 g. L-1 potassium) gave the highest percentages for plant height, number of main branches, leaf dry weight, number of total leaves, and total chlorophyll content, where recorded (89.68 cm, 12.67 main branches. plant-1, 48.43 g. plant-1, 189.09 leaves. plant-1, 12,609 mg. 100 g-1 fresh weight, respectively). It may be concluded that the application of an integrated fertilization program can sustain or enhance stevia production in both quantity and quality under challenging environmental circumstances.

The study was carried out in the canopy belongs to the Horticulture and Landscape Department - College of Agriculture - University of Kerbala in pots with a capacity of 28 kg during the spring growing season 2021 to know the effect of spraying with glutathione, biofertilizer (Algacell) under different water stress levels on the effectiveness of some enzymes and the content of some vitamins of Moringa Oleifera plant. The study was designed as a factorial experiment according to the randomized complete block design (R.C.B.D), as the study included three factors: glutathione with three concentrations (0, 100, 200) mg.L−1 and biofertilizer (Algacell) at two levels (0 and 10) ml. L−1 and water stress with three levels of field capacity (60%, 50%, 40%), and the averages were compared using Dunkin’s multinomial test at a probability level of 0.05. Most of the vegetative growth characteristics were the spraying treatment at a concentration of 200 ml. L−1 achieved the highest rates in terms of (the activity of the enzyme catalase, the activity of the dismutase enzyme, the activity of the peroxidase enzyme, the concentration of ascorbic acid), which reached (2.75%, 28.622%, 6.005 mg.gm−1, 7.561%, 0.377 units.gm−1, 11.777 gm−1, 0.990 gm−1, 275.444 mg.gm−1), respectively, followed by the spray treatment with a concentration of 100 ml. L−1, while the comparison treatment recorded fewer concentrations, while the bio-fertilizer (Algacell) did not achieve a significant difference in all the studied traits, while the water stress treatment excelled at the level of 40% of the field capacity in the traits (the activity of the catalase enzyme, the activity of the dismutase enzyme, the activity of the peroxidase enzyme, the concentration of ascorbic acid, the concentration of α-Tocopherol) which gave respectively (0.446 gm−1, 13.222 gm−1, 1.133 gm−1, 294.167 mg.gm−1, 257.83 μg.gm−1), and the effect of the double and triple interaction between the treatments was similar to their single effects in improving most of the studied traits.