SIPA1 (signal-induced proliferation-associated protein 1) is a GTPase activation protein that can catalyze the hydrolysis of Rap1 bound GTP to GDP. Recently attention has been paid to a potential role for SIPA1 in cancer metastasis; however, the underlying mechanism of how changes in SIPA1 levels may lead to increased metastasis remains poorly understood. In this study, we showed that SIPA1 was mainly localized to the nuclei in highly invasive breast cancer tumor tissue and MDA-MB-231 cells. Knockdown of SIPA1 in MDA-MB-231 altered cell morphology and cell proliferation ability. Furthermore, this study is the first to establish that nuclear SIPA1 can interact with the integrin β1 promoter and activate its transcription; this interaction appears to be important for SIPA1-dependent MDA-MB-231 cell adhesion and invasion. We also demonstrated that the phosphorylation of FAK, Akt and the expression of MMP9, downstream signaling molecules of integrin β1, were decreased upon SIPA1 knockdown, and MDA-MB-231 cell invasion was impaired. Taken together, these results suggest nuclear SIPA1 contributes to breast cancer cell invasion through the regulation of integrin β1 signaling.

Food-borne infections and diseases remain one of the major concerns of public health and food safety caused by a wide range of pathogens contaminating food and food products. Staphylococcal food poisoning is one of the most economically important foodborne diseases in public health programs worldwide while  the most common and important species of Escherichia genus which consists of five species,  these species that cause human infection. A total of 44 swab samples were collected from different animals (Cow, Buffalo, and Goat). These samples were collected from Al Qurnah farm, animal farm of Basrah veterinary collage. These samples were collected mainly from 4 locations in the animal body (nose, mouth, rectum, and vagina). The results indicates that the most E.  coli isolation comes from the cows followed by goat and the most contaminated areas was mouth ( 100 % ) followed by nasal cavity ( 90.9 % ) , rectum ( 72% ) and finally vaginal cavity (54.5 %) while the results of S. aureus infected revealed that high percentage , were rectal samples (90.9%); nasal samples; (81.8%)and (72.7%) for both vaginal and oral samples. All animals were infected approximately equally with S. aureus. Antibiotic susceptibility test for E. coli concluded that the bacteria were sensitive to (Clindamycin, Cefoxirim and Erythromycin) and were resistance to Vancomycin and Tetracycline While Antibiotic susceptibility result for S. aureus showed susceptibility to Clindamycin, and Tetracycline and complete resistance to Cefotaxime and Vancomycin.

Food-borne infections and diseases remain one of the major concerns of public health and food safety caused by a wide range of pathogens contaminating food and food products. Staphylococcal food poisoning is one of the most economically important foodborne diseases in public health programs worldwide while  the most common and important species of Escherichia genus which consists of five species,  these species that cause human infection. A total of 44 swab samples were collected from different animals (Cow, Buffalo, and Goat). These samples were collected from Al Qurnah farm, animal farm of Basrah veterinary collage. These samples were collected mainly from 4 locations in the animal body (nose, mouth, rectum, and vagina). The results indicates that the most E.  coli isolation comes from the cows followed by goat and the most contaminated areas was mouth ( 100 % ) followed by nasal cavity ( 90.9 % ) , rectum ( 72% ) and finally vaginal cavity (54.5 %) while the results of S. aureus infected revealed that high percentage , were rectal samples (90.9%); nasal samples; (81.8%)and (72.7%) for both vaginal and oral samples. All animals were infected approximately equally with S. aureus. Antibiotic susceptibility test for E. coli concluded that the bacteria were sensitive to (Clindamycin, Cefoxirim and Erythromycin) and were resistance to Vancomycin and Tetracycline While Antibiotic susceptibility result for S. aureus showed susceptibility to Clindamycin, and Tetracycline and complete resistance to Cefotaxime and Vancomycin.

This study was conducted in Basrah at period from August 2018 till February 2019. Six hindered (600) blood samples were collected from different fields of cattle suspected infected with LSD according to the clinical examination at Al Mudaina and Al Qurnah town, north of Basrah province to detect the antibodies against LSD (550 females, 50 males, 200 calves less than one year old, and 400 cows aged more than one year). The result revealed that, the overall prevalence of LSD in cattle was 18.66% ( 112 out of 600 ), the prevalence of disease in calves was 30% ( 60 out of 200 ) ;While in cow was 13% ( 52 out of 400 ), and there was significant differences in the prevalence of LSD between calves and cows ( P˂ 0.05 ). The prevalence of LSD in female was 16.3% (90 out of 550), while in male was 44% (22 out of 50), and there was significant differences in prevalence of LSD between males and females. The clinical study revealed that LSD was found in three clinical forms include nodular dermatitis observed in 104 cases (92.8%), arthritis in 20 cases (17.8%) and lymphadenopathy in 3 cases (2.5%).

Bacillus thuringiensis is a ubiquitous a gram-positive, anaerobic, spore-forming bacterium, produces various proteins (toxins) during the stationary and vegetative phase of its growth cycle. Some of these proteins belonging to this group showed weak similarities to ß-barrel pore-forming toxic proteins, such as the cytotoxins of Pseudomonas aeruginosa, the epsilon toxin of Clostridium perfringens, alpha-toxin of Clostridium septicum. In the present study, the intoxication effects of Cry51Aa1 protein produced by B.thuringiensis F14-1 strain was evaluated in vivo, in order to investigate the ability of activated Cry51Aa1 to make a change in size or functions of zebrafish cardiovascular system and induces toxicity in adult zebrafish. Using in vivo imaging we observed that Cry51Aa1 has no scientific effect on cardiac function and development of zebra fish embryo or have toxic effect on adult in spite of use high concentration, so it is safe to use and a potentially effective agent in breast cancer therapy.