A field experiment was conducted during the winter season 2018-2019 at Al- Qurna site to investigate the effect of row spacing and spraying of complete nano-fertilizer on growth and yield of Wheat (Triticum asetivum L.). The experiment was arranged in a split-plot by using R.C.B.D design with Three replicates, the main-plots were row Spacing (10, 20, and 30 cm), the sub-plots included Nano-fertilizer levels (0, 2.5, 3.5, and 4.5ml. L-1). Results showed that the row spacing gave higher value for most characters, the distance 30 cm rat gave plant height and flag leaf area, 1000 grain weight, grain yield, and bio yield, as they were (88.67cm, 46.02cm2,45.69g, 7.86 t. h-1, 21.37 t. h-1), respectively. Treatment of fertilization with nano spraying with a concentration of 4.5 ml. l-1 was significantly superior in most indicators of growth and yield, it gave the highest averages compared to other fertilizer levels in plant height, leaf area, weight of 1000 grains, grain yield, number of spikes, number of tillers reached (89.23 cm, 47.30 Cm2, 44.87 g, 7.96 t. ha-1, 650.22 spikes. m2, 750.56 tillers. m2) respectively. The interaction between factors was significant in most of the growth indicators in two components of yield (number of spikes and number of spike seeds) in addition to the biological yield.