"The study was conducted in the laboratories of Faculty of Agriculture/University of Kufa for the period from 24/12/2017 to 24/2/2019 to isolate and diagnose fungi associated with imported bananas fruits (Musa spp.) in local markets. The study included five varieties of bananas for different origins to identify the ability of isolated fungi and their production for mycotoxins, including F. proliferatum. and evaluation of the effectiveness ultraviolet radiation uv-c with a wavelength of 254 nm, for 15 minutes and different concentrations of peppermint and aloevera extracts were evaluated their effectiveness in reducing of Fumonisin B1. The results showed the effect of UV-C and different concentrations of hot water extracts of peppermint and aloe vera to inhibit growth fungus F. proliferatum on PSA medium, a significant effect of peppermint extract,as it showed an inhibition of the growth fungus F. proliferatum. Concentration 1.5%, with amounted to 75.52% compared to control treatment, in which the percentage of inhibition was 0.00%. Ultraviolet light (UV-c) had a significant role in inhibiting the growth of the fungus. Inhibition which increasing the duration of the fungus exposure to ultraviolet (UV) radiation, as the duration of 15 minutes gave the highest rate of inhibition amounting to 81.35 % compared to control treatment of 0.00 %. The results also showed the high efficiency of ultraviolet radiation and peppermint extract at concentration of 1.5% in reducing toxicity of Fumonisin B1 and the protecting banana fruits from fungal infection, in addition to the possibility of prolonging the storage period."
This study aimed to isolate and diagnose the main pathogens of zucchini root rot, test their pathogenicity, and evaluate their control with some biological agents under laboratory conditions. The results of diagnostic isolation from the roots of the zucchini crop that showed symptoms of the disease showed the presence and dominance of two isolates of Fusarium oxysporum (FO1, FO2), three isolates of Fusarium solani (FS1, FS2, FS3) and three isolates of Rhizoctonia solani (RS1, RS2, RS3) and two isolates of Pythium spp (PY1, PY2), which was present in a ratio of 65-25% in most of the samples collected from the fields of the Faculty of Agriculture/University of Karbala. F. solani had the highest frequency, at 60.00%, and had the highest occurrence in sample 3, which was 65.00%, followed by R. solani and F. oxysporum with 48.33 and 40.00% occurrence, respectively. The pathogenicity test of fungal isolates on red radish seeds showed that all isolates significantly reduced radish seeds germination under laboratory conditions. The results showed the effect of pathogenic fungal isolates on germination and growth of zucchini plants in protected cultivation, which led to a significant reduction in germination percentage, fresh weight of the plant, plant height and a high rate of disease severity. FS1 and RS1 isolates completely prevented seed germination, followed by FS2 and FO2 isolates with equal germination rate of 22.22%, compared to control treatment with 100% germination rate. FS2 isolate had the highest infection severity rate of 97%, followed by FO2 isolation with 94% compared to no infection in control treatment. The minimum plant length was 0.267 cm in the FS2 isolation treatment, followed by the FS3 isolate, with a plant length of 0.567 cm, compared to 2.553 cm in the control treatment. FO1 isolation led to the highest reduction in plant fresh weight by 81%, followed by isolate FS2 with 78% compared to control treatment. The test of the inhibitory ability of the biological control agents showed the presence of high antagonism of Trichoderma harzianum, Chaetomium sp and Sordaria sp against pathogenic fungal isolates. T. harzianum was the highest antagonistic against all the pathogenic fungal isolates, recording grade 1 antagonism according to Bell et al. (1982) after seven days of dual culture. Also, Chaetomium sp and Sordaria sp showed antagonistic ability of 1 and 2 degrees against pathogenic fungal isolates under study.
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