The current research aims at revealing the role of strategic alignment through its dimensions (communication, governance, infrastructure, partnership and skills) in achieving organizational excellence through its dimensions (excellence in leadership, excellence in service, excellence in knowledge through organizational ambidexterity). In order to achieve the goal of the research a number of colleges of Karbala University was selected. Thus, the research started from a problem expressed by a number of intellectual and practical questions aimed at answering. By diagnosing the level of its importance and its impact and the possibility of achieving strategic alignment in the colleges surveyed, it has used the research questionnaire and a way to get the data and use the research descriptive and analytical approach, which consists of three standards: strategic alignment, organizational excellence and organizational ambidexterity. It was used to consolidate and strengthen these concepts and focus on teachers; an intentional sample included 80 members of teaching colleges. The data were collected from the main sources of information using the questionnaire form; the data were analyzed using a set of statistical methods by means of software (SPSS V.23). The researchers reached a number of conclusions, the most prominent of which is that strategic alignment represents a set of behaviors and attributes that seek innovation and transformation to find new paths for exploring investment and energies available to them.