Aim Neonatal sepsis is a clinical syndrome of illness accompanied by bacteremia that develops in the first month of life. The objective of this study was to evaluate the reliability and quality of YouTube TM ( www.youtube.com ) videos pertaining neonatal sepsis. Methods The first 100 videos on YouTube TM pertaining to neonatal sepsis were included in the analysis. Features like videos’ image type, content and qualification of video creators were recorded. In addition, videos’ length, upload date, time since upload, comment and like counts were also recorded. Quality of the videos was measured by the researchers using the Global Quality Scale (GQS) and reliability of the videos was evaluated through the DISCERN (Quality Criteria for Consumer Health Information on Treatment Choices) tool. Results The total length of the examined 100 videos was 35.84 hours and the total view count was 1,173,247. The most common video content was general information about neonatal sepsis, education and diagnosis. When qualification of the video creators was examined, the most common creators were physicians followed by other persons and health channels. The videos were divided into two groups according to the creators. Accordingly, 40 (40%) videos were uploaded by professionals and 60 by non-professionals. There was a statistically significant difference between physicians and non-physicians in terms of the mean DISCERN and GQS scores (both, p<0.01). Conclusion Both DISCERN and GQS scores were statistically significantly higher in the videos provided by physicians. Physicians should be encouraged to upload accurate informative videos about neonatal sepsis and direct parents to accurate sources of treatment.