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Objective: The present work was conducted to explore the prevalence of Pseudomonas aeruginosa that produce lipase and protease enzymes in 40 samples, as well as detection of quality and quantity production and determine its susceptibility to antimicrobial. Methods:The antimicrobial activity of Lactococcus cell free supernatant (CFS) on P. aeruginosa growth and quantity production of enzymes by two methods (special media and OD) were also studied.Results: A total of 8(20%) and 13(32%) isolates were found to be positive to P. aeruginosa producing lipase and protease, respectively. The in vitro antimicrobial activity results revealed that all isolates producing enzymes showed sensitive to CFS of Lactococcus, only one isolate exhibited low sensitivity to CFS 4 mm, however, these isolates varied in their sensitivity to CFS ranged 4-15 mm. Conclusion:Results of the quantity production of P. aeruginosa enzymes with CFS of Lactococcus showed and exerts growth inhibitory activity and reduce the production of enzymes. We could be concluded that CFS of Lactococcus has great potential antimicrobial activity against P. aeruginosa growth and its ability on enzymes production.

Probiotic are alive microorganisms presented in food and dietary supplements, probiotics beneficially affect the individual by improving the intestinal microbial balance properties . This study revealed anew pathway of siderophore attenuation in Pseudomonas aeruginosa, using Lactobacillus. Out of forty P. aeruginosa isolates , 20 isolates were siderophore produced , Cell free supernatant (CFS) and Cell free culture (CFC) of Lactobacillus the antimicrobial agent were able to produce zones of inhibition against P. aeruginosa growth in average (8-15 mm) and (9-15 mm) by CFS and CFC respectively .These antimicrobial agent also were able to attenuate the pathogenicity of P. aeruginosa by prevent its ability to siderophore production.