EAEC is a diarrheal pathogen of emerging importance. Correlation between pCVD432 PCR and the HeLa cell line assay was confirmed in children with diarrhea. In comparison to the assay for aggregative adherence, the EAEC PCR has been found to be simple and specific in many epidemiological studies. The typical EAEC (73.3%) strains (with pCVD432 and aggR genes) identified in this study were heterogeneous with respect to virulence genes. This study also showed that EAEC isolates were highly resistant to tetracycline, co-trimoxazole, and ampicillin, which are the most commonly used antibiotics in our area.

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