The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent of those samples with SCC above 200,000 (n=13) were screened for real-time PCR identification of microorganisms and blood samples were genotyped for TLR4 SNPs. There was a higher prevalence of Gram-positive bacteria in the analyzed samples (88.9%) and animals that had the combined genotypes AACCCC, GGTCGG and GACCGC presented the lowest somatic cell scores, and consequently those genotypes have the potential to be applied as molecular markers for assisted animal selection to improve milk quality.

Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157®, bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.

Objetivo: Identificar a prevalência bacteriana em mão, estetoscópio e celulares de profissionais e acadêmicos de saúde em enfermarias de um hospital filantrópico em Anápolis, Goiás, avaliando-se o perfil de sensibilidade das bactérias isoladas a beta-lactâmicos e verificar a ocorrência de práticas de higienização para estetoscópios e ou celulares. Métodos: estudo observacional, transversal e descritivo. Os pesquisados foram submetidos à aplicação de questionário e à coleta de amostra biológica. As amostras foram testadas para a pesquisa de bactérias do gênero Staphylococcus spp e para bacilos gram-negativos com destaque para enterobactérias produtoras de beta-lactamase de espectro estendido (ESBL) e Staphylococcus aureus resistente à oxacilina (ORSA). Resultados: foram avaliadas 60 amostras de mãos, 59 celulares e 19 estetoscópios, observando-se contaminação de 86,7% amostras de mãos, 89,8% de celulares e 94,7% de estetoscópios. A maior prevalência de ORSA foi observada em amostras de estetoscópios (25%). Não foi documentado isolamento de ESBL. A não adesão à prática de higienização de estetoscópios e celulares foi verificada em 26,3% e 27,1% dos participantes, respectivamente. Observou-se que as mãos de estudantes de medicina, terapeutas e outros profissionais exibiram maior taxa de contaminação. Conclusões: foram verificadas altas taxas de contaminação em fômites e mãos de acadêmicos e profissionais de saúde em enfermarias. A importância conferida em relação à higienização das mãos é maior comparada aos fômites pesquisados.

HOMEOINDEX is a bibliographic database in which everything published in the field of homoeopathy is progressively filed. The methods adopted are those used by major international medical databases (MEDLINE, LILACS), allowing data exchange.HOMEOINDEX radically changes the situation for dissemination, study, preservation and research of homoeopathic works. It takes homoeopathy out of an archaic phase when work was inaccessible and fragmented and into the modern era.The development of this work included some unique achievements:—Introduction of homoeopathy as recognized literature in international institutions—Adoption of homoeopathic terms (key-words) in official latin-American medical catalogues—Creation of a Latin American reference centre, for cataloguing and disseminating homoeopathic literature—The introduction of homoeopathic papers to international medical databases.

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