The study of biocultural relations helps us understand how human societies have appropriated their territory, generating an interdependence that, through history and the current environmental crisis through which contemporary societies are passing, shows the imbalance in conceiving the relationship between nature and culture. This connection is essential for those who seek to generate strategies guaranteeing the sustainability of conservation processes in the territory. The study site was Basurú and Acosó, rural Istmina and Condoto, respectively. The overall objective of the research was to contribute to the identification and recognition of community biocultural knowledge as a strategy of permanence and self-management of their territories. This participatory research included: 1) identifying the main plant species used by the communities for daily activities, 2) characterization of their main uses and properties (medicinal, food, forestry, magic-religious, others), states of threat, associated activity, and modes of use. A qualitative methodology was used, including applying ethnographic techniques: open and semi-structured interviews, questionnaires in search of data on uses, properties, and associated activity, participant observation, guided excursions, and review of secondary information. It was determined that some of the daily use species had been introduced. Others are native, all classified according to their uses: medicinal, forest, food, magic-religious, and others. As a result, there is a direct association between the medicinal and food species, to a lesser extent the medicinal species with the magical-religious and finally the forest species. Most medicinal ones are used to treat respiratory affections, tropical diseases, deworming, midwifery, traumas and womb diseases.