Research on the production of biomass and lipid content of Leptolyngbya HS-16 grown in a photobioreactor with variations in the number of air bubbles forming holes in the photobioreactor has been carried out. Photobioreactor (PBR) is a system designed to support the life of microorganisms in the system by providing various factors that can be used for growth such as light, carbon dioxide and nutrients. Bubble column photobioreactor (BCPBR) provides aeration for mixing nutrients and a carbon dioxide source for culture. In this study, two types of bubble column photobioreactor (BCPBR) were used with variations in the number of air bubbles, namely 12 (BCPBR1) and 24 (BCPBR2). In addition, TPBR without aeration was used as a control. This study used Leptolyngbya HS-16, an indigenous cyanobacteria isolated from Red Crater, Pancar Mount, West Java. Leptolyngbya HS-16 was inoculated on a bubble column photobioreactor (BCPBR). The lipids obtained from this strain were 4.41% (BCPBR1) and 1.30% (BCPBR2) after 24 days.