No abstract

The aim of this research was producing model of autoimmune myocarditis induced by immunization with cardiac myosin and identification of acute stage of inflammation.We shown that single subcutaneous injection of cardiac myosin (400 μg/kg) combined with incomplete Freund's adjuvant (CFA) or CFA alone led to inflammatory response in rat myocardium with maximal reaction on 14–21 days after immunization. It was earlier confirmed by test of antibody levels in blood serum, light and electron microscopy of heart sections, expression of inflammatory cytokines and iNOS by RT‐PCR methods and analysis of nitrite and nitrate levels in blood serum and myocardial homogenates on 2, 3, 4, 7, 14, 21 and 28 days after immunization. We substituted CFA by incomplete Freund's adjuvant (IFA) without Mycobacterium tuberculosis wall components and repeated experiment and all testes on 4, 14 and 21 days. Our data shown that immunization of rats with myosin mixed with IFA caused the inflammatory response in myocardium on 4 day with maximum was detected on 21 day. In IFA injected rats the inflammation was also detected, but had a slow onset and developed to 21 day's.We examined two models of autoimmune myocarditis and shown that CFA and IFA can independently provoke inflammation in myocardium.