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Objectives: This study aims to verify whether red and infrared lasers can interfere with the healing process of wounds located at the point of application and at distance. Materials and methods: Three circular wounds were conducted on the dorsum of rats, being one in the cranial region, another one in the medial region, and a third one in the caudal region. For this experimental study, 45 animals were investigated and divided into three groups, to which 15 animals were submitted to no laser of any kind, 15 animals were submitted to infrared laser, and 15 animals were submitted to red laser. The observation period was of three, five, and seven days. Thus, each group was divided into three subgroups (A, B, C) based on the observation period. For groups II and III the amount of energy during the irradiation was 8J/cm 2 , applied after the surgery and then every 48 hours, totalizing, depending on the time of death of the animal, two, three or four irradiations. Results: There was higher presence of edema in among 3 and 5 day period of analysis and acute inflammatory process were found in red laser irradiation study group. Quantitatively, there was no statistically significant difference in the percentage of reduction of wounds in and among the groups. Conclusions: There is no statistical difference were found by the therapy with low power red and infra-red laser in the wound sizes during all period of analysis.

ResumoObjetivo: Incorporar o hormônio de crescimento recombinante humano em um polímero biodegradável (PLGA). Material e método: As matrizes foram confeccionadas através da técnica de evaporação de solventes. Foi feita uma mistura do polímero (poli ácido glicólico lático) e do hormônio do crescimento humano recombinante (Saizen  Merck Serono S.A. Aubonne, Suíça). Essa mistura foi vertida em moldes de silicone circulares de 1 cm de diâmetro e aproximadamente 2 mm de espessura, e levada para secagem em uma câmara de evaporação de solvente por 48 horas. Após esse período, as matrizes foram imersas em PBS e passaram por um banho termostatizado (ensaio de degradação hidrolítica), in vitro, à temperatura de 37°C. As amostras foram retiradas do banho no intervalo de 1, 2, 3, 4, 7, 10 e 14 dias. Foram aferidas a perda de massa, a variação do pH e a concentração do hormônio liberado em função do tempo. Resultado: A concentração do hormônio liberado em função do tempo foi aumentando até o terceiro dia. No quarto dia, houve uma queda e, no sétimo, ocorreu um aumento do hormônio liberado, estendendo-se até o décimo dia; no 14° dia, houve queda novamente. O pH teve uma queda brusca de 7,4 para 3,2 no primeiro dia, mantendo uma pequena queda até o 14° dia. A perda de massa foi gradual em relação ao tempo, como já era esperado. Conclusão: O PLGA é um bom biomaterial para confecção de matrizes com hormônio do crescimento. Revelou-se possível incorporar o rhGH nessa matriz, de modo a, então, desenvolver-se um substituto ósseo.Descritores: Polímeros; hormônio do crescimento; biodegradação. AbstractObjective: Incorporate recombinant human growth hormone in a biodegradable polymer (PLGA). Material and method: The arrays were fabricated by solvent evaporation technique. A mixture of polymer (poly lactic glycolic acid) and recombinant human growth (Saizen  Merck Serono SA Aubonne, Switzerland) was performed hormone. This mixture was poured into circular molds silicone 01cm in diameter and about 02mm thick, and carried into a drying chamber for evaporation of solvent for 48 hours. After this period, the matrices were immersed in PBS and passed through a constant temperature bath (test for hydrolytic degradation) in vitro, at a temperature of 37°C. The samples were removed from the bath in the range of 01, 02, 03, 04, 07, 10, 14 days. Mass loss, pH and concentration of hormone released as a function of time was measured. Result: The concentration of hormone released versus time was increased until the third day. On the fourth day had a fall and on the seventh day there have been increased hormone released by the tenth day, the fourteenth day was falling again. The pH had a sharp drop from 7.4 to 3.2 on the first day and keeping a small drop until the fourteenth day. The mass loss was a gradual loss in relation to time as was to be expected. Conclusion: PLGA is a good biomaterial for making breeders of growth hormone. It has proved possible to incorporate the rhGH in the array so as to then develop a bone substitute.Descriptors: Polym...

Residual cyst is an inflammatory odontogenic cyst resultant from pulp necrosis, where the tooth has already been removed. Bisphosphonates are drugs used in the prevention and treatment of bone metabolism diseases with intense resorption activity, and they have been associated with osteonecrosis of the jaws, an important side effect. The aim of this study was to report a successful surgical treatment of a residual cyst in a patient who had previously developed jaw osteonecrosis associated with sodium alendronate use. We emphasize here the importance of safe preoperative procedures when approaching these patients.